Product
소개
DNA Relaxation Assays Kit
Drug Target and Enzyme Assays
DNA topoisomerases such as bacterial topoisomerase I convert supercoiled circular DNA into relaxed DNA (DNA relaxation reaction).
Bacterial topoisomerase IV also has the DNA relaxation activity.
The DNA Topoisomerase I Assay is based on the principle that the relaxed DNA suppresses the fluorescent intensity much more than the supercoiled
DNA when the DNAinteracts with fluorescence dye H19 (a fluorescence dye for DNA relaxation / supercoiling assay) in the presence of magnesium.
When the supercoiled DNA is converted into its relaxed form, the fluorescent signal decreases.
Human Topoisomerase I DNA Relaxation Assay Kit Plus -100 (Catalog No. HRA100KE)
Kit components: includes 480 μl of 10 x Buffer HT1, 405 μl of 10 x supercoiled plasmid DNA, 10μl of 5000 x human topoisomerase I enzyme (500,000 U/ml), 20 μl of 1500 x Dye H19 and 3000 μl of 10 x H19 dilution buffer. It includes all the reagents for 100 DNA relaxation assays in a 96-well plate format or 200 assays in a 384-well assay format.
E. coli DNA Topoisomerase I Assay Kit Plus-100 (Catalog No. DRA100KE)
Kit components: 480μl10 x Buffer T1, 405μl10 x supercoiled DNA, 20μl1500 x Dye H19, 3000μl 10 x H19 dilution buffer, 42μl 100 x E coli Topo I.
E. coli Topo IV DNA Relaxation Assay Kit Plus (Catalog No. T4RA-100KE)
Kit components: 480μl 10 x Buffer T2, 405μl10 x supercoiled DNA, 20μl1500 x H19 dye, 3 ml 10 x H19 dilution buffer, 420μl10 mM ATP, 45μl100x E.coli Topo IV.
P. aeruginosa Topo IV DNA Relaxation Assay Kit Plus (Catalog No. T4RA-100KP)
Kit components: 480μl10 x Buffer T2, 405μl10 x supercoiled DNA, 20μl1500 x H19 dye, 3 ml 10 x H19 dilution buffer, 420μl10 mM ATP, 43μl100x P. aeruginosa Topo IV.
S. aureus Topo IV DNA Relaxation Assay Kit Plus (Catalog No. T4RA-100KS)
Kit components: 480μl10 x Buffer T2, 405μl10 x supercoiled DNA, 20μl1500 x H19 dye, 3 ml 10 x H19 dilution buffer, 420μl10 mM ATP, 43μl100 x S. aureus Topo IV.
S. pneumoniae Topo IV DNA Relaxation Assay Kit Plus (Catalog No. T4RA-100KN)
Kit components: 480μl10 x Buffer T2, 405μl10 x supercoiled DNA, 20μl1500 x H19 dye, 3 ml 10 x H19 dilution buffer, 420μl10 mM ATP, 43μl100x S. pneumoniae Topo IV.