Mobile Phase Additives and Ion Pairing Reagents

ProteoChem's common mobile phase additives and ion pairing reagents are used in HPLC and LCMS. Not all LCMS mobile phase reagents are the same however. Contaminated mobile phases can cause baseline inconsistencies and decrease UV signal and mass spectrometric signal.

ProteoChem’s Proteomics Grade mobile phase additives and ion pairing reagents are recommended for HPLC and LCMS applications where mobile phase purity is essential.

 

     Acetic Acid (Proteomics Grade) 

     Formic Acid (Proteomics Grade) 

     TFA (Proteomics Grade)

     HFBA (Proteomics Grade)

     Formic Acid + TFA (Proteomics Grade) 

 

1.  Acetic Acid (Proteomics Grade)

Acetic Acid is a common mobile phase additive used in HPLC and LCMS solvents. However, not all Acetic Acids are the same. Contaminated mobile phases can cause baseline inconsistencies, decrease UV signal, and mass spectrometric signal. ProteoChem’s Proteomics Grade Acetic Acid in ampules is > 99.8% pure and recommended for HPLC and LCMS applications where mobile phase solvent purity is essential.

Each LCMS Acetic Acid ampule contains 1.0 mL of Proteomics Grade Acetic Acid, making preparation of 0.1% v/v Acetic Acid a snap (1 ampule in 1 liter of solvent). Ten Acetic Acid Amps are sold in each package for all your LCMS and HPLC grade Acetic Acid needs.

•  Molecular Weight: 60.05

•  CAS Number: 64-19-7

•  Alternative Names: Ethanoic acid; Glacial Acetic acid

•  Note: Sold for nonmedical use only. Not to be used or sold as a drug for humans or animals. 

 

 

2.  Formic Acid (Proteomics Grade)

Formic Acid is a common mobile phase solvent additive used in many HPLC and LCMS applications. ProteoChem's Proteomics Grade Formic Acid in ampules is > 99.0% pure and is of exceptional quality. ProteoChem's LCMS Formic Acid amps are for HPLC and proteomics/mass spec applications (i.e. as an eluent additive for LCMS) and is made from HPLC grade Formic Acid pure enough for the most sensitive research purposes. ProteoChem's ampule-packed Formic Acid LCMS mobile phase solvent can help improve peak shape, increase peak resolution, and will improve signal in positive ion mass spectrometry ionization.

ProteoChem's LCMS Formic Acid ampules contain 1.0 mL of proteomics grade Formic Acid, making preparation of 0.1% v/v Formic Acid a snap (1 ampule in 1 liter of solvent). Ten Formic Acid amps are sold per package for all your LCMS and HPLC grade Formic Acid needs.

•  Molecular Weight: 46.03

•  CAS Number: 64-18-6

•  Alternative Names: Methanoic Acid; Formic Acid Amps

•  Note: Sold for nonmedical use only. Not to be used or sold as a drug for humans or animals. 

 

 

3.  TFA (Proteomics Grade)

Trifluoroacetic acid (TFA) is an effective ion pairing agent for HPLC and proteomics LCMS applications which are regularly used to resolve chromatographically similar analytes. In peptide separations, ProteoChem's LCMS grade TFA in amber glass ampules is proven to improve peak shape and resolution.

ProteoChem’s LCMS Trifluoroacetic Acid is among the highest purity LCMS TFA available (> 99.9% pure). The high purity and low absorbance cut-off of ProteoChem's LCMS grade TFA makes it ideal for preparing mobile phase solvents for HPLC and LCMS studies.

ProteoChem's Proteomics Grade LCMS TFA ampules contain 1.0 mL of proteomics grade Trifluoroacetic Acid, making preparation of 0.1% v/v Trifluoroacetic Acid a snap (1 ampule in 1 liter of solvent). Ten LCMS Trifluoroacetic Acid amps are sold per package for all your LCMS and HPLC grade Trifluoroacetic Acid needs. 

 

 

•  Molecular Weight: 114.03

•  CAS Number: 76-05-1

•  Alternative Names: TFA Ampoules; Trifluoroacetic Acid Ampoules; TFA Amps 

☞   Trifluoroacetic acid Product Information Sheet & General Protocol

 

 

4.  HFBA (Proteomics Grade)

Heptafluorobutyric Acid (HFBA) is an ion pairing agent for reverse-phase HPLC separation of peptides or proteins. ProteoChem's HFBA purity is > 99.5% to maximize sensitivity of your assay. HFBA provides sensitive low UV wavelength nondestructive peptide detection in reverse-phase HPLC protein and peptide separation systems. HFBA is packaged in 1 mL ampules for your convenience - It takes only seconds to prepare 1 liter of fresh 0.1% heptafluorobutyric acid solution mobile phase for reverse-phase chromatography.

ProteoChem’s HFBA is ideally suited as an ion pair reagent for reverse-phase HPLC applications. HFBA is excellent for protein/peptide sequencing and as a protein or peptide solubilizing reagent. HFBA is often used in amino acid analysis and solid-phase peptide synthesis. Our highly refined HFBA is tested for optimal HPLC performance. 

 

 

•  Molecular Formula: C4HF7O2

•  Molecular Weight: 214.04

•  Density: 1.64 g/mL

•  CAS Number: 375-22-4

•  Alternative Names: HFBA; Heptafluorobutanoic acid; Perfluorobutanoic acid; Perfluorobutyric acid; 2,2,3,3,4,4,4-Heptafluorobutanoic acid 

•  Note: Sold for nonmedical use only. Not to be used or sold as a drug for humans or animals.

 

 

☞   HFBA Product Information Sheet & General Protocol

5.  Formic Acid + TFA (Proteomics Grade)

A common mobile phase solvent used in peptide LCMS and other LCMS applications is 0.1% Formic Acid with 0.01% Trifluoroacetic acid (TFA). Formic Acid+TFA mobile phase solvent is intended to increase the MS signal obtained (versus TFA alone), while still obtaining the chromatographic benefits of TFA.

Each Formic Acid+TFA ampule contains 1.0 mL of Proteomics Grade Formic Acid (> 99.0% pure) and 0.1 mL of Proteomics Grade TFA (> 99.9% pure), making preparation of 1 liter of mobile phase at 0.1% v/v Formic Acid + 0.01% TFA a snap (1 ampule in 1 liter of solvent). This concentration of TFA and Formic Acid is ideal for giving adequate separation with minimal ion suppression due to ion pairing effects. Ten Formic Acid+TFA Amps are sold in each package. 

 

•  Molecular Weight: Mixture of Compounds

•  CAS Number: N/A

•  Note: Sold for nonmedical use only. Not to be used or sold as a drug for humans or animals. 

Cat. No	Product	Quantity
LC6200-10amps	Acetic Acid Ampules (Proteomics Grade)	10 x 1.0 mL
LC6201-10amps	Formic Acid Ampules (Proteomics Grade)	10 x 1.0 mL
LC6201-50mL	Formic Acid Ampules (Proteomics Grade)	50mL
LC6202-10amps	Formic Acid + TFA Ampules (Proteomics Grade)	10 x 1.0 mL
LC6203-10amps	TFA Ampules (Proteomics Grade)	10 x 1.0 mL
LC6203-10x1gm	TFA Ampules (Proteomics Grade)	10 x 1.0 gm
LC6206-10amps	HFBA (Proteomics Grade) 10 x 1.0 mL	10 x 1.0 mL