인성크로마텍 로고

검색버튼
퀵메뉴 펼침

o
p
e
n
c
l
o
s
e

Topoisomerase DNA decatenation assays
제품명: Topoisomerase DNA decatenation assays
용도: Drug Target and Enzyme Assays
메이커: ProFoldin
카달로그: 다운로드


소개

 

 

Topoisomerase DNA decatenation assays

 

Drug Target and Enzyme Assays

 

 

 

DNA topoisomerases such as bacterial DNA topoisomerase IV (the parC-parE complex) and human topoisomerase II convert the concatenated DNA into decatenated DNA during the DNA replication process in the cell. DNA decatenation activity by other topoisomerases including topoisomerase I and bacterial gyrases are also observed.


The Spin-column DNA Topoisomerase Decatenation Assaysare based on the principle that the decatenated DNA is separated from the concatenated DNA by a quick and easy spin-column process. The concatenated DNA stays on the column, while the decatenated DNA is eluted. The eluted DNA is quantified by fluorescence.

 


The 96-Well Topoisomerase DNA Decatenation Assay is in a 96-well assay plate format that can be used for high-throughput tests of topoisomerase inhibitors. The assay is based on the principle that the decatenated DNA is separated from the concatenated DNA by a filtration process. The decatenated DNA passed through the filter (TDD filter plate), received in a black 96 well plate and quantified by fluorescence at 535 nm (excitation at 485 nm).


 



Topoisomerase DNA decatenation assays.PNG

 

 

 


< Bacterial topoisomerase IV >

 


E. coli DNA Topoisomerase IV Assay Kit Plus-100 (Catalog No. DDC100KE)

Kit components: 400μl 10 x Buffer T4, 255μl 10 x concatenated DNA, 780μl20 x Dye, 550μl 0.5 M EDTA, 100 spin columns, 120μl 10 mM ATP, 55μl 100 x E.coli topo IV. 


It is for 100 assays in a 96-well plate format.

 

 


96-Well E. coli Topo IV DNA Decatenation Assay Kit Plus (Catalog No. EDD96KE)

Kit components: 600μl 10 x Buffer T2, 50μl 10x concatenated DNA, 530μl10 x fluorescence dye, 1 TDD plate,1 V-bottom plate, 2 ml 10 x Rinse buffer, 8μl1000 x E. coli topo IV, 120μl 10mM ATP, 500μlenzyme dilution buffer, 1 ml 0.4 M EDTA, 1 receiver plate. 


It is for 96 assays in a 96-well plate format.

 

 


96-Well Topoisomerase DNA Decatenation Assay Kit (Catalog No. TDD96K)

Kit components: 600μl 10 x Buffer T2, 500μl10x concatenated DNA, 260μl 20 x fluorescence dye, 1 TDD plate, 2 ml 10 x Rinse buffer, 110μl 10 mM ATP, 1 receiver plate, 1 V-bottom plate.


It is for 96 assays in a 96-well plate format.

 

 


96-Well P. aeruginosa Topo IV DNA Decatenation Assay Kit Plus (Catalog No. PDD96KE)

Kit components: 600μl 10 x Buffer T2, 500μl 10x concatenated DNA, 530μl 10 x fluorescence dye, 1 TDD plate,1 V-bottom plate, 2 ml 10 x Rinse buffer, 8 μl1000 x P. aeruginosa topo IV, 120μl 10 mM ATP, 500μl enzyme dilution buffer, 1 ml 0.4 M EDTA, 1 receiver plate. 


It is for 96 assays in a 96-well plate format.

 

 


96-Well S. aureus Topo IV DNA Decatenation Assay Kit Plus (Catalog No. SDD96KE)

Kit components: 600μl 10 x Buffer T2, 500μl 10x concatenated DNA, 530μl 10 x fluorescence dye, 1 TDD plate,1 V-bottom plate, 2 ml 10 x Rinse buffer, 8μl 1000 x S. aureus topo IV, 120 μl10 mM ATP, 500μl enzyme dilution buffer, 1 ml 0.4 M EDTA, 1 receiver plate. 


It is for 96 assays in a 96-well plate format.

 

 


96-Well S. pneumoniae Topo IV DNA Decatenation Assay Kit Plus (Catalog No. NDD96KE)


Kit components: 600μl 10 x Buffer T2, 500μl 10x concatenated DNA, 530μl 10 x fluorescence dye, 1 TDD plate,1 V-bottom plate, 2 ml 10 x Rinse buffer, 8μl 1000 x S. pneumoniae topo IV, 120μl 10 mM ATP, 500μlenzyme dilution buffer, 1 ml 0.4 M EDTA, 1 receiver plate. 


It is for 96 assays in a 96-well plate format.

 

 

 

 

 

< Human DNA topoisomerase II >


 

Human Topoisomerase II DNA Decatenation Assay Kit Plus-100 (Catalog No. HDC100KE)


Kit components: 550μl 10 x Buffer HT, 520μl 10 x concatenated DNA, 800μl 20 x Dye, 600μl0.5 M EDTA, 100 spin columns, 120μl 10 mM ATP, 7.5μl 10 U/ml human topo II, 1000μl enzyme dilution buffer. 


It is for 100 assays in a spin-column assay format.

 

 


96-Well Human Topo II DNA Decatenation Assay Kit Plus (Catalog No. HDD96KE)


Kit components: 600μl 10 x Buffer T2, 500μl 10x concatenated DNA, 530μl 10 x fluorescence dye, 1 TDD plate, 1 V-bottom plate, 2 ml 10 x Rinse buffer, 6μl 1000x human topo II, 120μl 10mM ATP, 500μl enzyme dilution buffer, 1 ml 0.4 M EDTA, 1 receiver plate . 


It is for 96 assays in a 96-well plate format.