Product
소개
Topoisomerase DNA decatenation assays
Drug Target and Enzyme Assays
DNA topoisomerases such as bacterial DNA topoisomerase IV (the parC-parE complex) and human topoisomerase II convert the concatenated DNA into decatenated DNA during the DNA replication process in the cell. DNA decatenation activity by other topoisomerases including topoisomerase I and bacterial gyrases are also observed.
The Spin-column DNA Topoisomerase Decatenation Assaysare based on the principle that the decatenated DNA is separated from the concatenated DNA by a quick and easy spin-column process. The concatenated DNA stays on the column, while the decatenated DNA is eluted. The eluted DNA is quantified by fluorescence.
The 96-Well Topoisomerase DNA Decatenation Assay is in a 96-well assay plate format that can be used for high-throughput tests of topoisomerase inhibitors. The assay is based on the principle that the decatenated DNA is separated from the concatenated DNA by a filtration process. The decatenated DNA passed through the filter (TDD filter plate), received in a black 96 well plate and quantified by fluorescence at 535 nm (excitation at 485 nm).
< Bacterial topoisomerase IV >
E. coli DNA Topoisomerase IV Assay Kit Plus-100 (Catalog No. DDC100KE)
Kit components: 400μl 10 x Buffer T4, 255μl 10 x concatenated DNA, 780μl20 x Dye, 550μl 0.5 M EDTA, 100 spin columns, 120μl 10 mM ATP, 55μl 100 x E.coli topo IV.
It is for 100 assays in a 96-well plate format.
96-Well E. coli Topo IV DNA Decatenation Assay Kit Plus (Catalog No. EDD96KE)
Kit components: 600μl 10 x Buffer T2, 50μl 10x concatenated DNA, 530μl10 x fluorescence dye, 1 TDD plate,1 V-bottom plate, 2 ml 10 x Rinse buffer, 8μl1000 x E. coli topo IV, 120μl 10mM ATP, 500μlenzyme dilution buffer, 1 ml 0.4 M EDTA, 1 receiver plate.
It is for 96 assays in a 96-well plate format.
96-Well Topoisomerase DNA Decatenation Assay Kit (Catalog No. TDD96K)
Kit components: 600μl 10 x Buffer T2, 500μl10x concatenated DNA, 260μl 20 x fluorescence dye, 1 TDD plate, 2 ml 10 x Rinse buffer, 110μl 10 mM ATP, 1 receiver plate, 1 V-bottom plate.
It is for 96 assays in a 96-well plate format.
96-Well P. aeruginosa Topo IV DNA Decatenation Assay Kit Plus (Catalog No. PDD96KE)
Kit components: 600μl 10 x Buffer T2, 500μl 10x concatenated DNA, 530μl 10 x fluorescence dye, 1 TDD plate,1 V-bottom plate, 2 ml 10 x Rinse buffer, 8 μl1000 x P. aeruginosa topo IV, 120μl 10 mM ATP, 500μl enzyme dilution buffer, 1 ml 0.4 M EDTA, 1 receiver plate.
It is for 96 assays in a 96-well plate format.
96-Well S. aureus Topo IV DNA Decatenation Assay Kit Plus (Catalog No. SDD96KE)
Kit components: 600μl 10 x Buffer T2, 500μl 10x concatenated DNA, 530μl 10 x fluorescence dye, 1 TDD plate,1 V-bottom plate, 2 ml 10 x Rinse buffer, 8μl 1000 x S. aureus topo IV, 120 μl10 mM ATP, 500μl enzyme dilution buffer, 1 ml 0.4 M EDTA, 1 receiver plate.
It is for 96 assays in a 96-well plate format.
96-Well S. pneumoniae Topo IV DNA Decatenation Assay Kit Plus (Catalog No. NDD96KE)
Kit components: 600μl 10 x Buffer T2, 500μl 10x concatenated DNA, 530μl 10 x fluorescence dye, 1 TDD plate,1 V-bottom plate, 2 ml 10 x Rinse buffer, 8μl 1000 x S. pneumoniae topo IV, 120μl 10 mM ATP, 500μlenzyme dilution buffer, 1 ml 0.4 M EDTA, 1 receiver plate.
It is for 96 assays in a 96-well plate format.
< Human DNA topoisomerase II >
Human Topoisomerase II DNA Decatenation Assay Kit Plus-100 (Catalog No. HDC100KE)
Kit components: 550μl 10 x Buffer HT, 520μl 10 x concatenated DNA, 800μl 20 x Dye, 600μl0.5 M EDTA, 100 spin columns, 120μl 10 mM ATP, 7.5μl 10 U/ml human topo II, 1000μl enzyme dilution buffer.
It is for 100 assays in a spin-column assay format.
96-Well Human Topo II DNA Decatenation Assay Kit Plus (Catalog No. HDD96KE)
Kit components: 600μl 10 x Buffer T2, 500μl 10x concatenated DNA, 530μl 10 x fluorescence dye, 1 TDD plate, 1 V-bottom plate, 2 ml 10 x Rinse buffer, 6μl 1000x human topo II, 120μl 10mM ATP, 500μl enzyme dilution buffer, 1 ml 0.4 M EDTA, 1 receiver plate .
It is for 96 assays in a 96-well plate format.