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Topoisomerase ATPase Assay Kit
제품명: Topoisomerase ATPase Assay Kit
용도: Drug Target and Enzyme Assays
메이커: ProFoldin
카달로그: 다운로드


소개

 

 

Topoisomerase ATPase Assays Kit

 

Drug Target and Enzyme Assays

 


 

Type II DNA topoisomerases including topoisomerase II and topoisomerase IV hydrolyze ATP as the source of molecular energy to carry out DNA supercoiling and DNA decatenation reactions. Inhibition of the ATPase activity of topoisomerases by compounds such as novobiocin blocks their biological functions. Therefore, the topoisomerase ATPase assay can be used for highthroughput screen of topoisomerase inhibitors. 


The Topoisomerase ATPase Assay is based on detection of the phosphate produced by the ATP hydrolysis reaction in the presence of DNA. 


The assay is in a 384-well plate format and the phosphate is detected using light absorbance at 650 nm.

 

 

 


Topoisomerase ATPase assays.PNG

 

 

 

 

 

 

E. coli gyrase ATPase assay Kit Plus (Catalog No. T2A-100KE)

Kit components: 400μl 10 x Buffer T2, 35μl 100 x DNA, 35μl 1000x ATP (20 mM), 33μl 100 x topoisomerase IV, 5 ml Dye. 


It is for 100 assays in a 384-well plate format.

 

 

 


Topoisomerase ATPase Assay Kit (Catalog No. TA1000)


Kit components: 4 ml 10 x Buffer T2, 320μl 100 x DNA, 320μl 100 x ATP (20 mM), 50 ml Dye.

It is for 1000 assays in a 384-well plate format.

 

 

 


S. aureus gyrase ATPase assay Kit Plus (Catalog No. T2A-100KS)


Kit components: 400μl 10 x Buffer T2, 35μl 100 x DNA, 35μl 1000x ATP (20 mM), 33μl 100 x S.aureus topoisomerase IV, 5 ml Dye. 


It is for 100 assays in a 384-well plate format.

 

 

 


E. coli Topo IV ATPase Assay Kit Plus (Catalog No. T4A-100KE)


Kit components: 400μl 10 x Buffer T2, 35μl 100 x DNA, 35μl 1000 x ATP (20 mM), 33μl 100 x E.coli topoisomerase IV, 5 ml Dye. 


It is for 100 assays in a 384-well plate format.

 

 

 


S. pneumoniae Topo IV ATPase Assay Kit Plus (Catalog No. T4A-100KN)


Kit components: 400μl 10 x Buffer T2, 35μl 100 x DNA, 35μl 1000x ATP (20 mM), 33μl 100 x S.pneumoniae topoisomerase IV, 5 ml Dye. 


It is for 100 assays in a 384-well plate format.

 

 

 


P. aeruginosa Topoisomerase IV ATPase Assay Kit Plus (Catalog No. T4A-100KP)


Kit components: 400μl 10 x Buffer T2, 35μl 100 x DNA, 35μl 1000x ATP (20 mM), 33μl 100 x P. aeruginosa topoisomerase IV, 5 ml Dye. 


It is for 100 assays in a 384-well plate format.

 

 

 


S. aureus Topoisomerase IV ATPase Assay Kit Plus (Catalog No. T4A-100KS)


Kit components: 400μl 10 x Buffer T2, 35μl 100 x DNA, 35μl 1000x ATP (20 mM), 33μl 100 x S. aureus topoisomerase IV, 5 ml Dye. 


It is for 100 assays in a 384-well plate format.