DNA Primase Assay Kit 

 

Drug target and enzyme assays

 

 

 

 

The bacterial DNA primase (DnaG) synthesizes RNA primers at the DNA replication fork where the DNA helicase (DnaB) unwinds the double strand DNA. 

The Bacterial DNA Primase Assays are based on measurement of the RNA primers synthesized by the DNA primase in the presence of the DNA temperate and NTPS. DNA helicases dramatically stimulate the activities of gram-negative bacterial primases (e.g. E. coli and H. influenzae) but not gram-positive bacterial primases (S. aureus and S. pneumoniae). 

The assays can be performed in a 96-well plate or 384-well plate format for high throughput screening of DNA primase inhibitors.

 

 

 

 

 

 

 

 

E. coli DNA Primase Assay Kit Plus-500 (E. coli primase-helicase included) (Catalog No. EGA500KE)

Kit components: 2500μl 10 x Buffer, 220μl 100 x DNA, 4500μl 10 x fluorescence dye, 220μl 100 x NTP, 220μl 100 x E. coli primase-helicase complex. 

It is for 500 assays in a 96-well plate format.

 

 

H. influenzae DNA Primase Assay Kit (enzyme not included) (Catalog No. HGA100K)

Kit components: 600μl 10 x Buffer, 45μl 100 x DNA, 850μl 10 x fluorescence dye, 45μl 100 x NTP. 

It is for 100 assays in a 96-well plate format.

 

H. influenzae DNA Primase Assay Kit Plus (enzyme included) (Catalog No. HGA100KE)

Kit components: 600μl 10 x Buffer, 45μl 100 x DNA, 850μl 10 x fluorescence dye, 45μl 100 x NTP, 45μl 100 x H.influenzae primase-helicase complex. 

It is for 100 assays in a 96-well plate format.

 

 

H. influenzae DNA Primase Assay Kit Plus-500 (enzyme included) (Catalog No. HGA500KE)

Kit components: 2500μl 10 x Buffer, 220μl 100 x DNA, 4500μl 10 x fluorescence dye, 220μl 100 x NTP, 220μl 100 x H.influenzae primase-helicase complex. 

It is for 500 assays in a 96-well plate format.

 

 

S. aureus DNA Primase Assay Kit (enzyme not included) (Catalog No. AGA100K)

Kit components: 600μl 10 x Buffer, 45μl 100 x DNA, 850μl 10 x fluorescence dye, 45μl 100 x NTP. 

It is for 100 assays in a 96-well plate format.

 

 

S. aureus DNA Primase Assay Kit Plus (enzyme included) (Catalog No. AGA100KE)

Kit components: 600μl 10 x Buffer, 45μl 100 x DNA, 850μl 10 x fluorescence dye, 45μl 100 x NTP, 45μl 100 x S. aureus primase. 

It is for 100 assays in a 96-well plate format.

 

S. aureus DNA Primase Assay Kit Plus-500 (enzyme included) (Catalog No. AGA500KE)

Kit components: 2500μl 10 x Buffer, 220μl 100 x DNA, 4500μl 10 x fluorescence dye, 220μl 100 x NTP, 220μl S. aureus 100x primase. 

It is for 500 assays in a 96-well plate format.

 

S. pneumonia DNA Primase Assay Kit (enzyme not included) (Catalog No. PGA100K)

Kit components: 600μl 10 x Buffer, 45μl 100 x DNA, 850μl 10 x fluorescence dye, 45μl 100 x NTP. 

It is for 100 assays in a 96-well plate format.

 

 

S. pneumonia DNA Primase Assay Kit Plus (enzyme included) (Catalog No. PGA100KE)

Kit components: 600μl 10 x Buffer, 45μl 100 x DNA, 850μl 10 x fluorescence dye, 45μl 100 x NTP, 45μl 100 x S. pneumoniae primase. 

It is for 100 assays in a 96-well plate format.

 

S. pneumonia DNA Primase Assay Kit Plus-500 (enzyme included) (Catalog No. PGA500KE)

Kit components: 2500μl 10 x Buffer, 220μl 100 x DNA, 4500μl 10 x fluorescence dye, 220μl 100 x NTP, 220μl 100 x S. pneumoniae primase. 

It is for 500 assays in a 96-well plate format.