DNA Helicase ATPase Assay Kit

 

Drug target and enzyme assays

 

 

 

DNA helicase (DnaB) hydrolyzes ATP as the source of molecular energy to carry out DNA unwinding required by the DNA replication process. Inhibition of the ATPase activity of DNA helicase blocks its DNA unwinding function. The DNA helicase ATPase assay can be used for high-throughput screen of DNA helicase inhibitors in drug discovery. 

The DNA Helicase ATPase Assay is based on detection of the phosphate produced by the ATP hydrolysis reaction in the presence of DNA. 

The assay is in a 384-well plate format and the phosphate is detected using light absorbance at 650 nm.

 

 

 

 

 

 

 

 

E. coli DNA Helicase ATPase assay kit Plus-100 (Catalog No. DNAB100KE)

Kit components: 500μl 10 x Buffer, 35μl 100 x DNA, 65μl 50 x E. coli Helicase, 35μl 100 x ATP, 5 ml dye. 

It is for 100 assays in a 384-well plate format.

 

 

E. coli DNA Helicase ATPase assay kit Plus-500 (Catalog No. DNAB500KE)

Kit components: 2 ml 10 x Buffer, 170μl 100 x DNA, 320μl 50 x E. coli Helicase, 170μl 100 x ATP, 25 ml dye. 

It is for 500 assays in a 384-well plate format.

 

P. aeruginosa DNA Helicase ATPase assay kit Plus-100 (Catalog No. DNAB100KP)

Kit components: 500μl 10 x Buffer, 35μl 100 x DNA, 65μl 50 x P. aeruginosa Helicase, 35μl 100 x ATP, 5 ml dye. 

It is for 100 assays in a 384-well plate format.

 

P. aeruginosa DNA Helicase ATPase assay kit Plus-500 (Catalog No. DNAB500KP)

Kit components: 2 ml 10 x Buffer, 170μl 100 x DNA, 320μl 50 x P. aeruginosa Helicase, 170μl 100 x ATP, 25 ml dye. 

It is for 500 assays in a 384-well plate format.

 

H. influenzae DNA Helicase ATPase assay kit Plus-100 (Catalog No. DNAB100KH)

Kit components: 500μl 10 x Buffer, 35μl 100 x DNA, 65μl 50 x H. influenzae Helicase, 35μl 100 x ATP, 5 ml dye. 

It is for 100 assays in a 384-well plate format.

 

H. influenzae DNA Helicase ATPase assay kit Plus-500 (Catalog No. DNAB500KH)

Kit components: 2 ml 10 x Buffer, 170μl 100 x DNA, 320μl 50 x H influenzae Helicase, 170μl 100x ATP, 25 ml dye. 

It is for 500 assays in a 384-well plate format.

 

S. pneumoniae DNA Helicase ATPase assay kit Plus-100 (Catalog No. DNAB100KN)

Kit components: 500μl 10 x Buffer, 35μl 100 x DNA, 65μl 50 x S. pneumoniae Helicase, 35μl 100x ATP, 5 ml dye. 

It is for 100 assays in a 384-well plate format.

 

S. pneumoniae DNA Helicase ATPase assay kit Plus-500 (Catalog No. DNAB500KN)

Kit components: 2 ml 10 x Buffer, 170μl 100 x DNA, 320μl 50 x S. pneumoniae Helicase, 170μl 100x ATP, 25 ml dye. 

It is for 500 assays in a 384-well plate format.

 

S. aureus DNA Helicase ATPase assay kit Plus-100 (Catalog No. DNAB100KS)

Kit components: 500μl 10 x Buffer, 35μl 100 x DNA, 65μl 50 x S. aureus Helicase, 35μl 100xATP, 5 ml dye. 

It is for 100 assays in a 384-well plate format.

 

S. aureus DNA Helicase ATPase assay kit Plus-500 (Catalog No. DNAB500KS)

Kit components: 2 ml 10 x Buffer, 170μl 100 x DNA, 320μl 50 x S. aureus Helicase, 170μl 100xATP, 25 ml dye. 

It is for 500 assays in a 384-well plate format.