DNA Liagase Assay Kit 

 

Drug target and enzyme assays

 

 

NAD+-dependent DNA ligases are present in bacteria, some entomopox viruses and mimi virus. Since NAD+-dependent DNA ligases are essential for bacterial growth, they are valuable targets for identifying novel antibacterial agents. 

The NAD+-dependent DNA Ligase Assay Kit is to measure the DNA ligase product in which the diphosphodiester bond is formed at the single stand break of a duplex DNA substrate. The ligase reaction is monitored by the fluorescence intensity at 535 nm. 

The assay is in 96-well plate format and can be used for screening inhibitors of DNA ligases from gram-positive (such as S. pneumoniae) and gram-negative (such as E. coli) bacteria.

 

 

 

 

 

 

 

 

NAD+-dependent DNA ligase assay kit (enzyme not included) (Catalog No. NLA100K)

Kit components: 800μl 10 x Buffer, 44μl 100x DNA, 220 ul 10 x dye, 21 ml Reagent T, 8μl 1000x NAD+. 

It is for 100 assays in a 96-well plate format.

 

 

E. coli NAD+-dependent DNA ligase assay kit Plus (E. coli ligase included) (Catalog No.NLA100KE)

Kit components: 800μl 10 x Buffer, 44μl 100 x DNA, 220μl 10 x dye, 21 ml Reagent T, 8μl 1000 x NAD+, 10 ul 500 x E. coli ligase. 

It is for 100 assays in a 96-well plate format.