MurA Assay Kit 

 

Drug target and enzyme assays

 

 

MurA or UDP-N-acetylglucosamine enolpyruvyl transferase catalyzes the first committed step in peptidoglycan biosynthesis in bacteria. It is an essential enzyme and attractive target for antibacterial drug discovery. MurA transfers enolpyruvate from phosphoenolpyruvate (PEP) to uridine diphospho-N-acetylglucosamine (UNAG) generating enolpyruvyl-UDPNacetylglucosamine (EP-UNAG) and inorganic phosphate. 

The MurA Assay is based on measurement of the inorganic phosphate generated from the MurA reaction. The inorganic phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of E.coli MurA in drug discovery research. It may also be used for characterization of E.coli MurA.

 

 

 

 

 

 

 

 

 

 

E. coli MurA Assay Kit Plus-100 (Catalog No. MURA100KE)

Kit components: 400μl 10 x Buffer, 35μl 100 x UGN, 35μl 100 x PEP, 35μl 100 x E. coli MurA, 5ml Dye MPA3000.

 

E. coli MurA Assay Kit Plus-500 (Catalog No. MURA500KE)

Kit components: 2 ml 10 x Buffer, 170μl 100 x UGN, 170μl 100 x PEP, 170μl 100 x E. coli MurA, 25 ml Dye MPA3000.