MurC Assay Kit 

 

Drug target and enzyme assays

 

 

MurC or UDP-N-acetylmuramic acid:L-alanine ligase is the first of four paralogous amino acidadding enzymes in the pathway of peptidoglycan biosynthesis in bacteria. It is an essential enzyme and attractive target for anti-bacterial drug discovery. MurC catalyzes the addition of Lalanine onto the nucleotide precursor UDP-MurNAc generating UDP-MurNAc-L-Ala. The ligation reaction is coupled to the hydrolysis of ATP forming ADP and inorganic phosphate. 

The MurC Assay is based on measurement of the inorganic phosphate generated from the MurC reaction. The inorganic phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette if a plate reader is not available. The high throughput assay can be used for screening inhibitors of MurC in drug discovery research. It may also be used for characterization of MurC.

 

 

 

 

 

 

 

 

E. coli MurC Assay Kit Plus-100 (Catalog No. MURC100KE)

Kit components: 400μl 10 x Buffer, 35μl 100 x UDP-MurAc, 35μl 100 x L-Ala, 35μl 100x ATP, 35μl 100x E. coli MurC, 5 ml Dye MPA3000.

 

E. coli MurC Assay Kit Plus-500 (Catalog No. MURC500KE)

Kit components: 2 ml 10 x Buffer, 170μl 100 x UDP-MurAc, 170μl 100 x L-Ala, 170μl 100x ATP, 170μl 100 x E. coli MurC, 25 ml Dye MPA3000.

 

S. pneumoniae MurC Assay Kit Plus-100 (Catalog No. MURC100KN)

Kit components: 400μl 10 x Buffer, 35μl 100 x UDP-MurAc, 35μl 100 x L-Ala, 35μl 100x ATP, 35μl 100 x S. pneumoniae MurC, 5 ml Dye MPA3000.

 

S. pneumoniae MurC Assay Kit Plus-500 (Catalog No. MURC500KN)

Kit components: 2 ml 10 x Buffer, 170μl 100 x UDP-MurAc, 170μl 100 x L-Ala, 170μl 100x ATP, 170μl 100 x S. pneumoniae MurC, 25 ml Dye MPA3000.