MurD Assay Kit

 

Drug target and enzyme assays

 

 

 

MurD is a D-Glutamic acid-adding enzyme in the pathway for bacterial cell-wall peptidoglycan synthesis. It is an essential enzyme and attractive target for anti-bacterial drug discovery. MurD catalyses the addition of D-glutamic acid to UDP-MurNAc-L-Ala, generating UDP-MurNAcdipeptide. The ligation reaction uses ATP hydrolysis as an energy source forming ADP and inorganic phosphate. 

The MurD Assay is based on measurement of the inorganic phosphate generated from the MurD reaction. The inorganic phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of MurD in drug discovery research. It may also be used for characterization of MurD.

 

 

 

 

 

 

 

 

E. coli MurD Assay Kit Plus-100 (Catalog No. MURD100KE)

Kit components: 400μl 10 x Buffer, 35μl 100 x UMA, 35μl 100 x D-Glu, 35μl 100 x ATP, 35μl 100 x E. coli MurD, 5 ml Dye MPA3000.

 

E. coli MurD Assay Kit Plus-500 (Catalog No. MURD500KE)

Kit components: 2 ml 10 x Buffer, 170μl 100 x UMA, 170μl 100 x D-Glu, 170μl 100 x ATP, 170μl 100 x E. coli MurD, 25 ml Dye MPA3000. 

 

P. aeruginosa MurD Assay Kit Plus-100 (Catalog No. MURD100KP)

Kit components: 400μl 10 x Buffer, 35μl 100 x UMA, 35μl 100 x D-Glu, 35μl 100 x ATP, 35μl 100 x P. aeruginosa MurD, 5 ml Dye MPA3000.

 

P. aeruginosa MurD Assay Kit Plus-500 (Catalog No. MURD500KP)

Kit components: 2 ml 10 x Buffer, 170μl 100 x UMA, 170μl 100 x D-Glu, 170μl 100 x ATP, 170μl 100x P. aeruginosa MurD, 25 ml Dye MPA3000.

 

 

S. aureus MurD Assay Kit Plus-100 (Catalog No. MURD100KS)

Kit components: 400μl 10 x Buffer, 35μl 100 x UMA, 35μl 100 x D-Glu, 35μl 100 x ATP, 35μl 100 x S. aureus MurD, 5 ml Dye MPA3000.

 

S. aureus MurD Assay Kit Plus-500 (Catalog No. MURD500KS)

Kit components: 2 ml 10 x Buffer, 170μl 100 x UMA, 170μl 100 x D-Glu, 170μl 100x ATP, 170μl 100 x S. aureus MurD, 25 ml Dye MPA3000.