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MurE Assay Kit
제품명: MurE Assay Kit
용도: Drug target and enzyme assays
메이커: ProFoldin
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소개

 

 

MurE Assay Kit 

 

Drug target and enzyme assays

 

 

 

MurE or UDP-MurNAc-tripeptide ligase is the third amino acid-adding enzymes in the pathway of peptidoglycan biosynthesis in bacteria . It is an essential enzyme and attractive target for anti-bacterial drug discovery. MurE catalyses the addition of lysine or meso-diaminopimelic acid (DAP) into the MurD product UDP-MurNAc-dipeptide in bacteria generating the UDP MurNActripeptide. The ligation reaction is coupled to the hydrolysis of ATP forming ADP and inorganic phosphate. 


The MurE Assay is based on measurement of the inorganic phosphate generated from the MurE reaction. The inorganic phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of MurE in drug discovery research. It may also be used for characterization of MurE.

 

 

 


 

MurE.PNG


 

 

 


 

E. coli MurE Assay Kit Plus-100 (Catalog No. MURE100KE)

Kit components: 400μl 10 x Buffer, 35μl 100 x DAP, 35μl 100 x UMAG, 35μl 100x ATP, 35μl 100 x E. coli MurE, 5 ml Dye MPA3000.




E. coli MurE Assay Kit Plus-500 (Catalog No. MURE500KE)

Kit components: 2 ml 10 x Buffer, 170μl 100 x DAP, 170μl 100 x UMAG, 170μl 100x ATP, 170μl 100 x E. coli MurE, 25 ml Dye MPA3000.