Bacterial Guanylate Kinase Assay Kit 

 

Drug target and enzyme assays

 

 

 

Guanylate kinase is essential for recycling GMP and cGMP. It catalyzes the ATP-dependent phosphorylation of GMP into GDP. Guanylate kinase is an attractive antibacterial target. 

The guanylate kinase assay is based on measurement of ADP generated from the kinase reaction. The assay is fluorescence-based and can be carried out using regular black 96-well or 384-well plates or cuvettes.

 

 

 

 

 

 

 

 

E. coli Guanylate Kinase Assay Kit Plus (Catalog No. GMK100KE)

Kit components: 600μl 10 x Buffer, 35μl 100x ATP, 35μl 100 x GMP, 5μl 1000 x E.coli GMK, 35μl 100 x MUK reagent A, 35l 100x MUK reagent B, 350μl 10 x fluorescence dye.

 

E. coli Guanylate Kinase Assay Kit Plus-500 (Catalog No. GMK500KE)

Kit components: 2 ml 10 x Buffer, 170μl 100x ATP, 170μl 100x GMP, 17μl 1000 x E.coli GMK, 170μl 100 x MUK reagent A, 170μl 100x MUK reagent B, 1700μl 10 x fluorescence dye.

S. pneumoniae Guanylate Kinase Assay Kit Plus-500 (Catalog No. GMK500KN)

Kit components: 2 ml 10 x Buffer, 170μl 100x ATP, 170μl 100x GMP, 17μl 1000x S.pneumoniae GMK, 170μl 100x MUK reagent A, 170μl 100x MUK reagent B, 1700μl 10 x fluorescence dye.