Universal Kinase Assay Kit 

 

Drug target and enzyme assays

 

 

The MicroMolar Universal Kinase Assay Kit is based on measurement of ADP generated from the kinase reaction. It is used to measure the activities of purified kinases producing ADP at concentrations ranging from 0.1 μM to 10 μM. 

The assay is fluorescence-based and can be carried out using regular black or white 96-well or 384-well plates or micro-cuvettes. It is important to avoid using unnecessarily high ATP concentrations in the kinase assays for the following reasons. 

One is to allow the assay to detect ATP-competitive inhibitors. The other is to avoid a high ADP background from ADP contamination in the ATP samples. It is recommended to use ATP concentrations at 20 μM (micromolar).

 

 

 

 

 

MicroMolar Universal Kinase Assay Kit (enzyme not included) (Catalog No. MUK100K)

Kit components: 600μl 10 x MUK Buffer, 35μl 10 mM ATP, 35μl 100x MUK reagent A, 35μl MUK reagent B, 350μl 10 x fluorescence dye, 30μl 1 mM ADP.

 

MicroMolar Universal Kinase Assay Kit-1000 (enzyme not included) (Catalog No. MUK1000K)

Kit components: 4 ml 10 x MUK Buffer, 350μl 10 mM ATP, 310μl 100x MUK reagent A, 310μl MUK reagent B, 3.5 ml 10 x fluorescence dye, 200μl 1 mM ADP.