Concentration assays for DNA, Protein, Peptide

 

 

 

 

1.  DNA

 

The NanoGram DNA Assay Reagent is for measurement of a wide range of DNA or RNA from nanograms to micrograms. 

The assay is based on the principle that DNA binds to the NanoGram fluorescence dye (Reagent D) and enhances the fluorescence intensity at 535 nm (excitation 485 nm).

 

 

 

 

 

NanoGram DNA Assay Reagent (Catalog No. NDA500)

Kit components: 5 ml 10 x Reagent D.

 

 

 

 

 

 

2.  Protein

 

The Easy Protein Assay Reagent provides a quick and simple method to measure protein concentrations. 

 

The assay is based on protein - Coomassie Blue binding and its light absorbance 595 nm. The assay is quick and easy: simply mix the diluted reagent with 1/10 volume of the protein solution and read the light absorbance at 595 nm. Standard clear 96-well plates or cuvettes can be used for absorbance reading.

 

 

 

 

 

 

Easy Protein Assay Reagent (Catalog No. EPA001)

Kit components: 100 ml 5 x reagent.

 

 

 

Environment-friendly SDS-PAGE staining

The Easy Protein SDS-PAGE Staining Solution is designed to avoid air pollution in the lab. The staining solution does not contain acetic acid or methanol. After a brief staining, the stained gel is simply destained in water. The gel staining is based on protein – Coomassie Blue binding that gives blue color.

 

 

 

 

 

 

Easy Protein SDS-PAGE staining solution (Catalog No. EPS001)

 

Kit components: 950 ml staining solution.

 

 

 

 

 

 

 

3.  Peptide

 

Short amino acid chains or peptides play key roles in many biological functions. Peptides from antigens are responsible for immune responses. Many biologically secreted and synthetic peptides have been used or being developed as drugs in various therapeutic areas including treatment of infection, cancer, diabetes, and cardiovascular diseases. The MicroMolar Peptide Assay Kit (Catalog No PEP200) is designed for concentration measurement of various peptides. 

The assay is based on increase of fluorescence at 535 nm of the dye C57 in the presence of peptides. The assay kit can be used for measurements peptide concentrations in synthetic or biochemical reactions, pharmaceutical products and environmental water samples.

 

 

 

 

 

 

 

MicroMolar Peptide Assay Kit (Catalog No PEP200)

 

Provides the reagent for measurement of 200 samples using 96-well plates. Cuvettes may also be used for measurements. The assay sensitivity varies from micromolar to millimolar concentrations depending on the nature of peptides. The assay is compatible with HEPES buffer, low concentrations of non-ionic detergent (<0.01%), MgCl2 (< 5 mM), CaCl2 (<5 mM), EDTA (< 1 mM) and phosphate (< 1 mM). It is not compatible with thiol compounds such as DTT.