Concentration assays for Detergent / SDS

 

 

 

 

1.  Detergent

 

The Detergent Assay Kit is for detection of various detergents at concentrations below their CMC values for most detergents. The concentration response is different from detergent to another. 

The assay is based on the principle that the detergent interacts with dye A43 and enhances the fluorescence intensity at 535 nm (excitation at 485 nm). The assay is compatible with most buffers and salts. It is not compatible with phosphate. Molecules such as ATP or ADP that release phosphate may interfere with the assay.

 

 

 

 

 

 

 

 

 

Detergent assay kit (Catalog No. DAK1000)

Kit components: 1 ml 100 x dye A43, 100 ml Reagent 1, 0.5 ml 100 x Reagent 2

 

 

 

 

 

 

2.  SDS

 

SDS (sodium dodecyl sulfate or sodium lauryl sulfate) is a common detergent and proteindenaturant. In laboratories, SDS is broadly used in cell lysis, protein analysis (SDS-PAGE) and membrane protein folding studies. In industry, SDS is used as a highly effective surfactant and isa key component in many cleaning products. SDS strongly binds to proteins and changes the protein conformation that affects the protein function. Complete removal of SDS in a proteinsolution is essential to fully recover the protein function and stability. The NanoGram SDS AssayKit (Catalog No SDS200) is designed for measurement of nanograms of SDS. The assay sensitivity is 0.002% SDS which is about 100 fold lower than its CMC value. 

The assay is based on increase of light absorbance at 650 nm of the dye MPS6 in the presence of SDS. The assay kit can be used for measurements SDS concentrations in samples with or without proteins. The assay is compatible with most biochemical buffers. It is not compatible with phosphate buffers.Inorganic phosphate interferes with the assay.

 

 

 

 

 

 

 

 

NanoGram SDS Assay Kit (Catalog No. SDS200)

Kit components: 20 ml MPS6 Dye.