Product
소개
D-Alanine Ligase Assay Kit
Drug target and enzyme assays
D-Alanine-D-Alanine is one of the building blocks in peptidoglycan biosynthesis in bacteria. This dipeptide is generated by ligation between two D-Alanine molecules catalyzed by D-Alanine : DAlanine ligase. The ligation reaction is coupled to the hydrolysis of ATP forming ADP and inorganic phosphate.
The E. coli D-Alanine :D-Alanine Ligase Assay is based on measurement of the inorganic phosphate generated from the D-Alanine : D-Alanine ligation reaction. The inorganic phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of E.coli D-Alanine : D-Alanine ligase in drug discovery research. It may also be used for characterization of E.coli D-Alanine : DAlanine ligase.
E. coli D-alanine ligase Assay Kit Plus-100 (Catalog No. DDA100KE)
Kit components: 400μl 10 x Buffer, 35μl 100 x DAA, 35μl 100 x ATP, 35μl 100x E. coli Ddl, 5 ml Dye MPA3000.
E. coli D-alanine ligase Assay Kit Plus-500 (Catalog No. DDA500KE)
Kit components: 2 ml 10 x Buffer, 170μl 100 x DAA, 170μl 100 x ATP, 170μl 100x E. coli Ddl, 25ml Dye MPA3000.