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D-Alanine Ligase Assay Kit
제품명: D-Alanine Ligase Assay Kit
용도: Drug target and enzyme assays
메이커: ProFoldin
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소개

 

 

D-Alanine Ligase Assay Kit 

 

Drug target and enzyme assays

 

 


D-Alanine-D-Alanine is one of the building blocks in peptidoglycan biosynthesis in bacteria. This dipeptide is generated by ligation between two D-Alanine molecules catalyzed by D-Alanine : DAlanine ligase. The ligation reaction is coupled to the hydrolysis of ATP forming ADP and inorganic phosphate. 


The E. coli D-Alanine :D-Alanine Ligase Assay is based on measurement of the inorganic phosphate generated from the D-Alanine : D-Alanine ligation reaction. The inorganic phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of E.coli D-Alanine : D-Alanine ligase in drug discovery research. It may also be used for characterization of E.coli D-Alanine : DAlanine ligase.

 

 

 

D-Alanine Ligase.PNG

 

 

 

 

 

 

E. coli D-alanine ligase Assay Kit Plus-100 (Catalog No. DDA100KE)

Kit components: 400μl 10 x Buffer, 35μl 100 x DAA, 35μl 100 x ATP, 35μl 100x E. coli Ddl, 5 ml Dye MPA3000.



 

E. coli D-alanine ligase Assay Kit Plus-500 (Catalog No. DDA500KE)

Kit components: 2 ml 10 x Buffer, 170μl 100 x DAA, 170μl 100 x ATP, 170μl 100x E. coli Ddl, 25ml Dye MPA3000.