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E. coli GlmU Assay Kit
제품명: E. coli GlmU Assay Kit
용도: Drug target and enzyme assays
메이커: ProFoldin
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소개

 

 

E.coli GlmU Assay Kit

 

Drug target and enzyme assays

 

 

 

UDP-N-acetylglucosamine pyrophosphorylase (GlmU) is a bifunctional enzyme that catalyzes transfer of acetyl and uridyl groups onto glucosamine-1-P to generate UDP-GlcNAc, an essential precursor of peptidoglycans. UDP-GlcNAc is involved in the synthesis of the Nacetylglucosamine polysaccharide adhesin required for biofilm formation in bacteria. 


The E. coli UDP-N acetylglucosamine pyrophosphorylase (GlmU) Assay is based on measurement of the pyrophosphate generated from the GlmU reaction. The pyrophosphate is converted to phosphate by pyrophosphatase and the phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. 


Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of E.coli GlmU in drug discovery research. It may also be used for characterization of E.coli GlmU.

 

 

 

UDP-N-acetylglucosamine pyrophosphorylase (GlmU) assays.PNG

 

 

 

 

 

 

E. coli GlmU Assay Kit Plus-100 (Catalog No. GLU100KE)

Kit components: 400μl 10 x Buffer, 35μl 100 x G-1-P, 35μl 100 x Ac-CoA, 35μl 100 x ppase, 35μl 100x E. coli GlmU, 5 ml Dye MPA3000.

 

 


 

E. coli GlmU Assay Kit Plus-500 (Catalog No. GLU500KE)

Kit components: 2 ml10 x Buffer, 170μl 100 x G-1-P, 170μl 100 x Ac-CoA, 170μl 100x ppase, 170μl 100x E. coli GlmU, 25 ml Dye MPA3000.