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Nicotinate phosphoribosyltransferase (pncB) Assay Kit
제품명: Nicotinate phosphoribosyltransferase (pncB) Assay Kit
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메이커: ProFoldin
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소개

 

 

Nicotinate phosphoribosyltransferase (pncB) Assay Kit

 

Drug target and enzyme assays

 

 


Nicotinate phosphoribosyltransferase (NAPRTase or PNCB) provides a pathway of regenerating nicotinic acid adenine dinucleotide (NAD) through synthesizing nicotinic acid mononucleotide (NAMN). The enzyme transfers nicotinate (NA) onto phosphoribosyl pyrophosphate (PRPP) releasing pyrophosphate. PNCB is drug target for gram (+) bacteria. 


The Nicotinate Phosphoribosyltransferase (PNCB) Assay is based on measurement of the pyrophosphate generated from the enzyme reaction. The pyrophosphate is converted to phosphate by pyrophosphatase and the phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. 


Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of PNCB in drug discovery research. It may also be used for characterization of PNCB enzyme.

 

 

 

Nicotinate phosphoribosyltransferase (pncB).PNG

 

 

 

 

 

 

S. pneumonia PncB Assay Kit Plus-100 (Catalog No. PNCB100KN)

Kit components: 400 μl 10 x Buffer, 35 μl 100 x PRPP, 35 μl 100 x NA, 35 μl 100x ATP, 35 μl 100xPpase, 35 μl 100x S. pneumoniae pncB, 5 ml Dye MPA3000.



S. pneumonia PncB Assay Kit Plus-500 (Catalog No. PNCB500KN)

Kit components: 2 ml 10 x Buffer, 170 μl 100 x PRPP, 170 μl 100 x NA, 170 μl 100x ATP, 170 μl 100x Ppase, 170 μl 100x S. pneumoniae pncB, 25 ml Dye MPA3000 .



S. aureus PncB Assay Kit Plus-100 (Catalog No. PNCB100KS)

Kit components: 400 μl 10 x Buffer, 35 μl 100 x PRPP, 35 μl 100 x NA, 35 μl 100x ATP, 35 μl 100xPpase, 35 μl 100x S.aureus pncB, 5 ml Dye MPA3000.



S. aureus PncB Assay Kit Plus-500 (Catalog No. PNCB500KS)

Kit components: 2 ml 10 x Buffer, 170 μl 100 x PRPP, 170 μl 100 x NA, 170 μl 100x ATP, 170 μl 100x Ppase, 170 μl 100x S. aureus pncB, 25 ml Dye MPA3000.