Methylerythritol phosphate cytidyltransferase (IspD)

 

Drug target and enzyme assays

 

 

 

Methylerythritol phosphate cytidyltransferase (IspD) is one of the enzymes in the nonmevalonate pathway for isoprenoid biosynthesis that present in many pathogenic organisms and plants but absent in mammals. IspD is an attractive target for the development of novel antibiotics and herbicides. This enzyme catalyzes the formation of 4-diphosphocytidyl-2-Cmethyl- D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP). 

The Methylerythritol Phosphate Cytidyltransferase (IspD) Assay is based on measurement of the pyrophosphate generated from the IspD reaction. The pyrophosphate is converted to phosphate by pyrophosphatase and the phosphate is detected by light absorbance at 650 nm. The assay is in a 384-well or 96-well plate format. It can be used for high throughput screening in drug discovery.

 

 

 

 

 

 

 

E. coli IspD Assay Kit Plus-100 (Catalog No. ISPD100KE)

Kit components: 400 μl 10 x Buffer, 35 μl 100 x MEP, 35 μl 100 x CTP, 35 μl 100x ATP, 35 μl 100xPpase, 35 μl 100x E. coli IspD, 5 ml Dye MPA3000.

E. coli IspD Assay Kit Plus-500 (Catalog No. ISPD500KE)

Kit components: 2 ml10 x Buffer, 170 μl 100 x MEP, 170 μl 100 x CTP, 170 μl 100x ATP, 170 μl 100x Ppase, 170 μl ul 100x E. coli IspD, 5 ml Dye MPA3000.

P. aeruginosa IspD Assay Kit Plus-100 (Catalog No. ISPD100KP)

Kit components: 400 ul 10 x Buffer, 35 μl 100 x MEP, 35 μl 100 x CTP, 35 μl 100x ATP, 35 μl 100xPpase, 35 μl 100x P. aeruginosa IspD, 5 ml Dye MPA3000.

P. aeruginosa IspD Assay Kit Plus-500 (Catalog No. ISPD500KP)

Kit components: 2 ml10 x Buffer, 170 μl 100 x MEP, 170 μl 100 x CTP, 170 μl 100x ATP, 170 μl 100x Ppase, 170 μl 100x P. aeruginosa IspD, 5 ml Dye MPA3000.