인성크로마텍 로고

검색버튼
퀵메뉴 펼침

o
p
e
n
c
l
o
s
e

Biotin Protein Ligase (BirA) Assay Kit
제품명: Biotin Protein Ligase (BirA) Assay Kit
용도: Drug target and enzyme assays
메이커: ProFoldin
카달로그:


소개

 

 

Biotin Protein Ligase (BirA) Assay Kit

 

Drug target and enzyme assays

 



Biotin protein ligase (BPL or BirA) is responsible for biotinylation of biotin-dependent proteins. It is an essential enzyme and an attractive target for anti-bacterial drug discovery. 


The E. coli Biotin Protein Ligase Assay is based on measurement of the pyrophosphate generated from the BirA reaction using hydroxylamine in the place of protein as a biotin receptor. The pyrophosphate is converted to phosphate by pyrophosphatase and the phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. 


Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of E.coli BirA in drug discovery research. It may also be used for characterization of E.coli BirA.

 

 

 

Biotin protein ligase (BirA) assays.PNG

 

 

 

 

 

 

E. coli Biotin protein ligase assay kit plus (Catalog No. BPL100KE)

Kit components: 400 μl 10 x Buffer, 35 μl 100 x biotin, 35 μl 100 x ATP, 35 ul 100 x hydroxylamine hydrochloride, 35 μl 100 x ppase, 35 μl 100 x E. coli BirA, 5 ml MPA3000.