Protein Folding Columns for Folding Non-Membrane Proteins

 

 

 

ProFoldin protein folding columns are designed to produce active proteins from guanidine hydrochloride or urea-solubilized inclusion bodies or protein aggregates formed during protein expression, purification or storage. Different proteins are folded under different conditions. 

The Spin-column Protein Folding Screen Kit includes 10 spin-columns that represent 10 optimized and diversified protein folding conditions including conditions allowing disulfide bond formation and reducing conditions. The folded protein samples from the screen kit are used for SDS-PAGE and activity tests. 

Based on the test results, the optimal condition (the column number) is selected for preparative folding.

 

 

 

 

 

 

 

 

Spin-Column Protein Folding Screen Kit (Catalog No. SFC01-10) 

Contains 0.16 ml Solution A, 0.6 ml Solution B, 10 spin columns.

 

 

 

 

 

 

 

Preparative protein folding column sets

 

The Preparative Protein Folding Column Sets are used for preparative protein folding after the folding condition has been identified by the Spin-column Protein Folding Screen Kit (Catalog No SFC01-10). The column number represents the specific folding condition.

 

 

Small-Scale Preparative Protein Folding Column Set (Catalog No. SFC01 to SFC10) 

Contains 0.16 ml Solution A, 0.6 ml Solution B, 10 spin columns. Each column set includes 10 identical preparative protein folding columns and reagents for folding 0.5 to 1 mg of denatured proteins.

 

Large-Scale Preparative Protein Folding Column Set (Catalog No. PFC01) 

Contains 5.4 ml Solution A, 8.4 ml Solution B, 14.4 ml Solution C, 4 preparative columns. The column set includes 4 identical preparative protein folding columns and reagents for folding 10 to 20 mg of guanidine hydrochloride or urea-solubilized protein.