AlbuVoid™      Albumin Depletion Plus Low Abundance Serum Protein Enrichment

 

•  Albumin voids in flow-through >95%, with <30 minute bind/wash/elute protocol

•  Low abundance enrichment equivalent or better than hexa-peptides or 

   antibodies

•  Disposable, cost-effective, no column regeneration or cross-contamination

•  Mild elution maintains tertiary structure and simple transfer to secondary 

   analysis

•  The eluted fractions retain their enzymatic and biological activity

•  Removes albumin from many species including human, mouse, sheep, 

   bovine, goat, rat, calf, cell culture.

•  The eluted fraction is compatible with LC-MS, activity based protein profiling 

   and proteomic studies.

 

AlbuVoid™ is a albumin depletion reagent kit. It removes albumin from serum and plasma samples while concentrating low abundance, and/or low molecular weight proteins. The AlbuVoid™ protocol uses mild buffers; the protocol conditions are so gentle that native enzyme activity is retained in elution fractions. AlbuVoid™ considerably enhances resolution of proteins below 50 kD, a limitation of alternate enrichment protocols.

AlbuVoid™ does not bind albumin. All other proteins(except albumin) in the sample binds to AlbuVoid™ and then you can elute off all the proteins minus the albumin. Resulting in low abundance serum protein enrichment. It is ideal for applications involving biomarker discovery, enzyme assays, toxicological studies for new drugs, protein profiling using SELDI analysis, protein array pixelation ,1D and 2D gel electrophoresis, LC/MS, and MALDI-TOF MS and cytokines research.

 

AlbuVoid™ derives from a silica-based library of individual mixed-mode polymeric ligands. The library was designed to facilitate weak binding of proteins, allowing for rapid elution from the matrix without any foreknowledge of the variety of proteins contained in the starting sample. Because of its specific binding properties, AlbuVoid™ depletes high abundance proteins in serum like albumin while improving the resolution of less abundant serum proteins.

 

Biotech Support Group has AlbuVoid™ for albumin depletion plus low abundance serum protein enrichment. Both Albusorb™ and AlbuVoid™ are available in AlbuTrial™ kit™. AlbuTrial™ kit™ includes 1 gm of Albusorb™ and 5 Preps of AlbuVoid™ with respective buffers.

 

 

☞   Click Here For AlbuVoid™ Product Sheet.

 

 

 

 

References

Application Report

Haiyan Zheng ¹; Swapan Roy²; Devjit Roy MD²; Amenah Soherwardy¹; Seema Rathman²; Matthew Kuruc². ¹Rutgers Center for Integrative Proteomics, Piscataway, NJ; ²Biotech Support Group LLC, Monmouth Junction, NJ.

Stroma Liquid Biopsy – Pan-Cancer Dysregulation of the Serum Proteome.

Synovial Fluid

Mihara, Keisuke, et al. "Identification of Specific Protein Markers of Rheumatoid Arthritis in Synovial Fluid and Serum." Journal of Hard Tissue Biology 27.1 (2018): 55-58.

Serum

Vialaret, Jerome & Kadi, Sarah & Tiers, Laurent & O Flynn, Robin & Lehmann, Sylvain & Hirtz, Christophe. (2018). Albumin depletion of human serum to improve quantitative clinical proteomics. Current Topics in Peptide & Protein Research 19. 53-62.

Roy, Swapan, and Matthew Kuruc. "Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors." Functional Proteomics. Humana Press, New York, NY, 2019. 41-54

Vialaret, Jerome, et al. "Albumin depletion of human serum to improve quantitative clinical proteomics."

Poillet-Perez, Laura, et al. "Autophagy maintains tumour growth through circulating arginine." Nature (2018): 1.

Mihara, Keisuke, et al. "Identification of Specific Protein Markers of Rheumatoid Arthritis in Synovial Fluid and Serum." Journal of Hard Tissue Biology 27.1 (2018): 55-58.

David L. Wang, Chuanguang Xiao, Guofeng Fu, Xing Wang and Liang Li. Identification of potential serum biomarkers for breast cancer using a functional proteomics technology. Biomarker Research (2017) 5:11. DOI 10.1186/s40364-017-0092-9

Swapan Roy, Matthew Kuruc. The Functional Subproteomes of Serpin Protease Inhibitors are Now Open for LC-MS Biomarker Discovery. MOJ Proteomics Bioinform 2016, 3(6): 00106

Sun, Zhenyu, Xiaofeng Chen, Gan Wang, Liang Li, Guofeng Fu, Matthew Kuruc, and Xing Wang. "Identification of functional metabolic biomarkers from lung cancer patient serum using PEP technology." Biomarker Research 4, no. 1 (2016): 1.

Haiyan Zheng; Caifeng Zhao; Swapan Roy; Devjit Roy MD; Amenah Soherwardy; Ravish Amin; Matthew Kuruc. Application Report - The Comparison of the Serum Proteome in Individuals with Cancers versus those without Cancer, and its application to Wellness

Dr.Swapan Roy; Amenah Soherwardy; Ravish Amin; Matthew Kuruc.Monmouth Junction, NJ - Application Report. AlbuSorb™ Product Extension combines Albumin and Immunoglobulin Depletion in a Consumable Format

Poster Reprint First Presented At AACR Annual Meeting 2016 Conference, Held April 17-20, 2016 New Orleans, LA USA.

The Commonality of the Cancer Serum Proteome Phenotype as analyzed by LC-MS/MS, and Its Application to Monitor Dysregulated Wellness Haiyan Zheng1; Caifeng Zhao1; Swapan Roy2; Devjit Roy MD; Amenah Soherwardy2; Ravish Amin2; Matthew Kuruc21Rutgers Center for Integrative Proteomics, Piscataway, NJ; 2Biotech Support Group LLC, Monmouth Junction, NJ

Xing Wang, Michael Davies, Swapan Roy and Matthew Kuruc. Bead Based Proteome Enrichment Enhances Features of the Protein Elution Plate (PEP) for Functional Proteomic Profiling . Proteomes 2015, 3(4), 454-466 . doi: 10.3390/proteomes3040454

Haiyan Zheng, Caifeng Zhao, Meiqian Qian, Swapan Roy, Absari Arpa, Amenah Soherwardy and Matthew Kuruc. AlbuVoid™ Coupled to On-Bead Digestion - Tackling the Challenges of Serum Proteomics. J Proteomics Bioinform 2015, 8:9.

Kuruc Matt, Dr.Roy. Application Report - AlbuVoid™ LC-MS On-Bead – Differential Expression of Cancer Sera Proteins Using Quantitative (iTRAQ) Proteomics

Grubbs, J. K., et al. " Investigation of the efficacy of albumin removal procedures on porcine serum proteome profile1." (2015).

Serum Profiling Making Mark on Predictive Medicine Vicki Glaser.Genetic Engineering & Biotechnology News. 2011;31(7):1-55.

Plasma

Espes, Daniel, Joey Lau, and Per-Ola Carlsson. " Increased circulating levels of betatrophin in individuals with long-standing type 1 diabetes." Diabetologia(2013): 1-4.

Patent - Secretome Sample Preparation

Narain, Niven Rajin, and Paula Patricia Narain. " COMPOSITIONS AND METHODS FOR DIAGNOSIS AND TREATMENT OF PERVASIVE DEVELOPMENTAL DISORDER." U.S. Patent No. 20,150,023,949. 22 Jan. 2015.

Narain, Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas. " INTERROGATORY CELL-BASED ASSAYS AND USES THEREOF." U.S. Patent No. 20,120,258,874. 11 Oct. 2012.

Patent - Serum Containing Medium

Narain, Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas . "Interrogatory cell-based assays for identifying drug-induced toxicity markers." U.S. Patent Application No. 16/180,446.

Cell Culture

Narain, Niven Rajin, Rangaprasad Sarangarajan, Vivek K. Vishnudas, and Michael Andrew Kiebish. " USE OF MARKERS IN THE IDENTIFICATION OF CARDIOTOXIC AGENTS AND IN THE DIAGNOSIS AND MONITORING OF CARDIOMYOPATHY AND CARDIOVASCULAR DISEASE." U.S. Patent 20,140,100,128, issued April 10, 2014.

On-Bead Digestion Protocols For LC-MS Proteomic Workflows

New on-bead digestion for LC-MS applications for proteomic studies

US HUPO 2014. Frontiers in Proteomics: Advancing Biology through Technology and Computation.

AlbuVoid™ abstract entitled "Improved proteomic enrichment and workflow strategies", poster board 089 presented at US HUPO 2014