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AlbuVoid™ PLUS
제품명: AlbuVoid™ PLUS
용도: Albumin & IgG Removal for Proteomics
메이커: Biotech Support Group
카달로그:


소개


AlbuVoid™ PLUS    Albumin and IgG Depletion From Serum/Plasma for Proteomics

 

•  IgG removal >90% (70-80% of total Immunoglobulins removed)AlbuVoid Plus.PNG

•  Albumin removal >95%

•  Seamless and simple < 1 hour protocol

•  Low abundance enrichment equivalent to immuno-affinity

•  Disposable, cost-effective, no column regeneration or cross-contamination

•  Works for most species tested including human, sheep, rat, mouse, bovine

•  On-bead protocols improve workflow and efficiency, especially suited to 

   targeted proteomics

•  Suitable for LC-MS, 1 and 2D Gels, ELISAs, Enzyme and other Functional 

   Assays.

 

The classical plasma proteins generally fall into functional categories, forming 

the vast majority of the mid-to-high abundance proteome. In serum, these 

sub-proteomes by mass content are: Albumin 50-60%; Immunoglobulins 10-20%; 

Transport Proteins (Transferrin, Apo) 5-10%; Complement related Proteins 3-5%; 

Protease Inhibitors 2-5%; and all others 2-5%. While these sub-proteomes are 

required for normal body homeostasis, they nevertheless can become dysfunctional 

during acute-phase and chronic stimuli.


So, depending on the needs of the investigation, it can be valuable to consider that one or more of these categorical sub-proteomes is simply background noise whereby depletion is beneficial. While in other cases, these same categorical sub-proteomes might provide new data and information and consequently, should not be depleted. Different AlbuVoid™, AlbuVoid™ PLUS and AlbuSorb™ PLUS workflows support different proteomic biases as outlined in the following Table.


Products and digest conditions produce different sub-proteome windows of observation. So, depending on the needs of the investigation, it can be valuable to consider that one or more of these categorical sub-proteomes is simply background noise whereby depletion is beneficial. While in other cases, these same categorical sub-proteomes might provide new data and information and consequently, should not be depleted. Categorically the acute-phase sub-proteomes differentiated in disease may vary greatly from those associated with chronic sub-proteomes. So there is great benefit in having options to enrich or deplete one or more of these sub-proteomes.


BSG's Albumin and IgG Removal Kits offer many such options:


•  The 'PLUS' products substantially deplete Immunoglobulins through separations at the protein level.

•  The variable regions of Immunoglobulins are extremely heterogeneous, generating a background noise across the full LC gradient. 

   On-bead digestion (BASP™) with AlbuVoid™ substantially reduces the influence of such Ig peptide features. So in addition to 

   workflow simplicity, BASP™ can be advantageous utilized in targeted proteomic workflows whenever the target proteins do not 

   require strong denaturing conditions.

•  With the exception of Immunoglobulins whereby FASP generates many more spectral features, both strong denaturing conditions 

   (FASP) and on-bead digest (BASP™) conditions produce similar protein profiles.

•  Both Apolipoproteins and heavily glycosylated proteins (i.e., α1-Acid Glycoprotein) bind poorly to AlbuVoid™. 

   For quantitative studies within these classes of proteins, AlbuSorb™ PLUS is recommended.

•  The Complement sub-proteome is especially enriched by AlbuVoid™ PLUS. The digest conditions may bias towards one or more 

   functional sub-populations, likely due to conformational transitions and protein-protein interactions (i.e., Factor Bb, Properdin) 

   that occur upon activation. This needs further investigation.

•  The low abundance sub-proteome is enriched 5+ fold with AlbuVoid™ and 4+ fold with AlbuSorb™ PLUS. 

 

☞  Click Here For The AlbuVoid™ Plus Product Sheet. 


 

3. AlbuVoid Plus_프로토콜.PNG


사양

3. AlbuVoid Plus_규격.PNG

주문정보

3. AlbuVoid Plus_주문정보.PNG

 

Kit Components : 


3. AlbuVoid Plus_주문정보_2.PNG

관련자료

References


AlbuVoid™ PLUS & AlbuSorb™ PLUS - Evaluating Different Windows of Observation Solves The Many Challenges of Serum Proteomics


Zheng H, Soherwardy A, Roy S, Kuruc M, Avadhani S. AlbuVoid™ Enrichment & Antibody Depletion – Tackling the Challenges of Serum Proteomics Part II. Poster reprint first presented at 26th International Molecular Med TRI-CON, March 10-15, 2019, San Francisco, CA USA


Vialaret, Jerome & Kadi, Sarah & Tiers, Laurent & O Flynn, Robin & Lehmann, Sylvain & Hirtz, Christophe. (2018). Albumin depletion of human serum to improve quantitative clinical proteomics. Current Topics in Peptide & Protein Research 19. 53-62.


Poillet-Perez, Laura, et al. "Autophagy maintains tumour growth through circulating arginine." Nature (2018): 1.


Roy, Swapan, and Matthew Kuruc. "Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors." Functional Proteomics. Humana Press, New York, NY, 2019. 41-54.


Mihara, Keisuke, et al. "Identification of Specific Protein Markers of Rheumatoid Arthritis in Synovial Fluid and Serum." Journal of Hard Tissue Biology 27.1 (2018): 55-58.


David L. Wang, Chuanguang Xiao, Guofeng Fu, Xing Wang and Liang Li. Identification of potential serum biomarkers for breast cancer using a functional proteomics technology. Biomarker Research (2017) 5:11. DOI 10.1186/s40364-017-0092-9


Zheng, H., et al. "AlbuVoid™ coupled to on-bead digestion-tackling the challenges of serum proteomics." J Proteom Bioinformatics 8.9 (2015): 225.


Zheng, H., et al. Poster reprint first presented at 14th Human Proteome Organization World Congress (HUPO 2015), held on September 27 – 30, 2015 entitled “New Proteomic Workflows Combine Albumin Depletion and On Bead Digestion, for Quantitative Cancer Serum”.