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Cleanascite™
제품명: Cleanascite™
용도: Lipid Removal & Clarification
메이커: Biotech Support Group
카달로그:


소개

 

Cleanascite™     Lipid Removal Reagent and Clarification13. Cleanascite.PNG


Cleanascite™ Lipid Removal Reagent and Clarification


•  Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon)

•  Workflows for antibodies, proteins, nucleic acids, proteoglycans, and serum 

   analytes

•  A high binding capacity for lipids with minimal cross-reactivity with proteins

   and nucleic acids

•  Ideal for clarifying ascites, serum, cell & tissue culture, bile and organ 

   homogenates

•  Clarifies saliva and fecal components

•  Exquisite selectivity profile including extracellular vesicle and exosome 

   clearance

•  Extends the life of membrane and chromatography columns.

•  Enrichment of delipidated tissue samples.

•  For downstream processing of large-scale therapeutic proteins, enzymes 

   and monoclonal antibodies.

  

Cleanascite™ selectively removes lipids, cell debris, lipoproteins, floating fats, impurities from cohn paste, transgenic milk, egg yolk and biological samples for pretreatment of samples prior to purification.  


The reagent is a solid-phase, non-ionic adsorbent supplied as a suspension in saline, ready for use. Simply add, centrifuge and/or filter. The clarified supernatant is ready for subsequent downstream processing or analysis. 

 

 

☞   Click Here To View Cleanascite™ Product Sheet

☞   Click Here To View Cleanascite™ Lipid Removal & Cell Response 

     Applications 

 


사양

13. Cleanascite_사양.PNG

주문정보

13. Cleanascite_주문정보.PNG

관련자료

References

Omental conditioned medium (OCM)

Chen, Rain R., et al. "Targeting of lipid metabolism with a metabolic inhibitor cocktail eradicates peritoneal metastases in ovarian cancer cells." Communications Biology 2 (2019).

Astrocytes


Nguyen, Amanda. "HDLs and LDLs in the Brain: Characterizing the Lipid Secretome of Astrocytes." The FASEB Journal 31.1_supplement (2017): 948-3.


Human serum, mouse serum or DMEM

 

Lee, Hong-Jai, et al. "Regulatory effect of humoral milieu on the viral DNA and surface antigen expression of hepatitis B virus (HBV) in vitro. " Molecular & Cellular Toxicology 15.2 (2019): 123-128.


Noncirculating Biological Fluids


Sprenkle, Neil T., et al. "Endoplasmic reticulum stress is transmissible in vitro between cells of the central nervous system." Journal of neurochemistry 148.4 (2019): 516-530.

Whole mouse serum

Dean, E. Danielle, et al. "Interrupted glucagon signaling reveals hepatic α cell axis and role for L-glutamine in α cell proliferation." Cell metabolism 25.6 (2017): 1362-1373.

 

Plasma

Swertfeger, Debi K., et al. "Feasibility of a Plasma Bioassay to Assess Oxidative Protection of Low Density Lipoproteins by High Density Lipoproteins." Journal of Clinical Lipidology (2018).


Estrous sheep serum

Barrera, Natalibeth, Pedro C. dos Santos Neto, Federico Cuadro, Diego Bosolasco, Ana P. Mulet, Martina Crispo, and Alejo Menchaca. "Impact of delipidated estrous sheep serum supplementation on in vitro maturation, cryotolerance and endoplasmic reticulum stress gene expression of sheep oocytes." PloS one 13, no. 6 (2018): e0198742.


Extracellular vesicles (EVs) from bone marrow (BM)-derived mesenchymal stromal cells (BM-MSC)

Nguyen, Doan C., Holly C. Lewis, Chester Joyner, Vivien Warren, Haopeng Xiao, Haydn T. Kissick, Ronghu Wu, Jacques Galipeau, and F. Eun-Hyung Lee. " Extracellular vesicles from bone marrow-derived mesenchymal stromal cells support ex vivo survival of human antibody secreting cells." Journal of extracellular vesicles 7, no. 1 (2018): 1463778.


Cell wall preparations

Li, J. (2015). Isolation and Preliminary Characterization of Anti-Inflammatory Molecules from the Staphylococcal Cell Wall (Doctoral dissertation, McGill University).


Pancreatic Rough Endoplasmic Reticulum (RER) Fractions

Waldron, R. T., Su, H. Y., Piplani, H., Capri, J., Whitelegge, J. P., Faull, K. F., ... & Freeman, M. R. (2018). Ethanol Induced Disordering of Pancreatic Acinar Cell Endoplasmic Reticulum: An ER Stress/Defective Unfolded Protein Response Model. Cellular and Molecular Gastroenterology and Hepatology , 5(4), 479-497.


Cells and bacterial lysates

Ahmed, Syed Mukhtar, and Ian G. Macara. "The Par3 polarity protein is an exocyst receptor essential for mammary cell survival." Nature Communications 8 (2017).

Human SZ95 sebocytes

Lovászi, M., et al. "Sebum lipids influence macrophage polarization and activation." British Journal of Dermatology (2017). doi: 10.1111/bjd.15754


Ascites Fluid

Chan, DW, Mak, SL, Ngan, HYS. The significance of lipid metabolism in peritoneal metastases of ovarian cancer. The 2016 Cold Spring Harbour Asia Conference on Cancer and Metabolism, Suzhou, China, 19-23 September 2016http://hub.hku.hk/handle/10722/235385

Serum

Taylor, Steven W., et al. "A high-throughput mass spectrometry assay to simultaneously measure intact insulin and C-peptide." Clinica Chimica Acta (2016).

Serum or Blood

Berggren, Per Olof, and Shao-Nian Yang. "Methods for treating and/or limiting development of diabetes." U.S. Patent Application No. 14/995,341.


Bile

Vesterhus, Mette, et al. "Novel serum and bile protein markers predict primary sclerosing cholangitis disease severity and prognosis." Journal of hepatology 66.6 (2017): 1214-1222.

Hauser-Davis, Rachel Ann. "Fish Bile Clean-up for Subsequent Zymography and Mass Spectrometry Proteomic Analyses." (2018).

Vesterhus, Mette, et al. " Novel serum and bile protein markers predict primary sclerosing cholangitis disease severity and prognosis." Journal of Hepatology 66.6 (2017): 1214-1222.

Laohaviroj, Marut, et al. " A comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma." Tumor Biology 39.6 (2017): 1010428317705764.

Vesterhus, Mette, Anders Holm, Johannes Roksund Hov, Ståle Nygård, Erik Schrumpf, Espen Melum, Liv Wenche Thorbjørnsen et al. " Novel serum and bile protein markers predict primary sclerosing cholangitis disease severity and prognosis." Journal of Hepatology (2017).

Megger, Dominik A., et al. "One Sample, One Shot-Evaluation of sample preparation protocols for the mass spectrometric proteome analysis of human bile fluid without extensive fractionation." Journal of Proteomics (2016).

Lukic, Natalija, et al. " An integrated approach for comparative proteomic analysis of human bile reveals overexpressed cancer-associated proteins in malignant biliary stenosis." Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1844.5 (2014): 1026-1033.

Danese, Elisa, et al. " Assessment of bile and serum mucin5AC in cholangiocarcinoma: Diagnostic performance and biologic significance."Surgery (2014).

Farina, Annarita, et al. " Bile carcinoembryonic cell adhesion molecule 6 (CEAM6) as a biomarker of malignant biliary stenoses." Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics (2013).

Boschetti, Egisto, and Pier Giorgio Righetti. Low-Abundance Proteome Discovery: State of the Art and Protocols. Newnes, 2013.

Wang W, Ai KX, Yuan Z, Huang XY, Zhang HZ. Different Expression of S100A8 in Malignant and Benign Gallbladder Diseases.Digestive diseases and sciences. 2012.

Hauser-Davis RA, Lima AA, Ziolli RL, Campos RC. First-time report of metalloproteinases in fish bile and their potential as bioindicators regarding environmental contamination. Aquatic Toxicology.2012;110-111:99-106

Farina A, Dumonceau JM, Frossard JL. Proteomic Analysis of Human Bile from Malignant Biliary Stenosis Induced by Pancreatic Cancer Journal of Proteome Research.2009; 8(1):159-69

Guerrier L, Claverol S, Finzi L et al. Contribution of solid-phase hexapeptide ligand libraries to the repertoire of human bile proteins. Journal of Chromatography.2007;1176(1-2):192-205

Chen Bo, Zheng Jian-wei, Wang Jian-ming, et al. Establishment and preliminary analysis of a 2-D human biliary map Chinese Journal of Hepatobiliary Surgery.2007

Chen B, Dong JQ, Chen YJ et al Two-dimensional electrophoresis for comparative proteomic analysis of human bile. Hepatobiliary & pancreatic diseases international.2007 Aug;6(4):402-6

Guerrier L, Claverol S, Finzi L et al Contribution of solid-phase hexapeptide ligand libraries to the repertoire of human bile proteins.Journal of Chromatography A.2007;1176(1-2):192-205

Kristiansen TZ, Bunkenborg J, Gronborg M et al A Proteomic Analysis of Human Bile Molecular and Cellular Proteomics.2004;3:715-728


Biological Matrices

Graeme T Clark, Paul J Russell, and Steven Westwood. Modification without impact: a case study in clinical assay failure due to lipemia. Bioanalysis; 2012: 4,(12):1421-1428


Egg Yolk

Ben Wade, Michelle Cummins, Anthony Keyburn and Tamsyn M. Crowley. Isolation and detection of microRNA from the egg of chickens. BMC Research Notes 2016 9:283.


Organ Homogenates

Myerson, J., He, L., Lanza, G., Tollefsen, D. and Wickline, S. Thrombin-inhibiting perfluorocarbon nanoparticles provide a novel strategy for the treatment and magnetic resonance imaging of acute thrombosis. Journal of Thrombosis and Haemostasis.2011;9:1292-1300

Thakuria D, Schmidt O, Liliensiek AK. Field preservation and DNA extraction methods for intestinal microbial diversity analysis in earthwormsJournal of Microbiological Methods.2009;76(3):226-33

Cheng AM, Moore EE, Masuno T et al Normal Mesenteric Lymph Blunts the Pulmonary Inflammatory Response to Endotoxin. Journal of Surgical Research.2006;136(S2):166-171

McNally T, Mackie IJ, Machin SJ et al. Increased levels of beta 2 glycoprotein I antigen and beta 2 glycoprotein I binding antibodies are associated with a history of thromboembolic complications in patients with SLE and primary antiphospholipid syndrome British journal of rheumatology.1995 Nov;34(11):1031-6


Red Blood Cells

Antunes RF; Brandao C; Maia M; Arosa FA. Red blood cells release factors with growth and survival bioactivities for normal and leukemic T cells. Immunology and Cell Biology.2011;89(1):111-21


Tracheal Swab Samples

Li D, Wang J, Wang R, Li Y. A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1, Biosensors and Bioelectronics.2011;26(S10):4146-4154

Fu LM, Shinnick TM. Genome-wide exploration of the drug action of capreomycin on Mycobacterium tuberculosis using Affymetrix oligonucleotide GeneChips Journal of Infection.2007;54(S3):277-284

Fu LM, Shinnick TM. Genome-wide analysis of intergenic regions of mycobacterium tuberculosis H37Rv using affymetrix genechips. EURASIP journal on bioinformatics & systems biology.2007:23054


Tissue and Cell Culture

United States Patent Application 20170348398 entitled: " COMPOSITIONS AND METHODS FOR DECREASING BLOOD GLUCAGON LEVELS

Ahmed, Syed Mukhtar, and Ian G. Macara. " The Par3 polarity protein is an exocyst receptor essential for mammary cell survival." Nature Communications 8 (2017): 14867. doi:10.1038/ncomms14867

Alhamdani MS, Schroder C, Hoheisel JD. Analysis conditions for proteomic profiling of mammalian tissue and cell extracts with antibody microarrays. Proteomics.2010;10(17):3203-7

Czambel RK, Kharlamov A, Jones SC. Variations of brain endothelial nitric oxide synthase concentration in rat and mouse cortex.Nitric Oxide.2010;22(S1): 51-57


Plasma/Serum

McIntyre, John A., et al. " Antiphospholipid autoantibodies as blood biomarkers for detection of early stage Alzheimer's disease." Autoimmunity0 (2015): 1-8.

Knight, Jason S., Wei Luo, Alexander A. O'Dell, Srilakshmi Yalavarthi, Wenpu Zhao, Venkataraman Subramanian, Chiao Guo et al. " Peptidylarginine Deiminase Inhibition Reduces Vascular Damage and Modulates Innate Immune Responses in Murine Models of Atherosclerosis." Circulation research (2014): CIRCRESAHA-113.

Palekar, Rohun U., et al. " Thrombin-Targeted Liposomes Establish A Sustained Localized Anticlotting Barrier Against Acute Thrombosis." Molecular pharmaceutics (2013).

Lijowski M, Caruthers S, Hu G. High-Resolution SPECT-CT/MR Molecular Imaging of Angiogenesis in the Vx2 Model Investigative Radiology.2009;44(1): 15–22

Turner JD, Langley RS, Johnston KL. Wolbachia Lipoprotein Stimulates Innate and Adaptive Immunity through Toll-like Receptors 2 and 6 to Induce Disease Manifestations of Filariasis The Journal of Biological Chemistry.2009;284:22364-22378

Torrelles JB, DesJardin LE, MacNeil J. et al Inactivation of Mycobacterium tuberculosis mannosyltransferase pimB reduces the cell wall lipoarabinomannan and lipomannan content and increases the rate of bacterial-induced human macrophage cell death Glycobiology.2009;19(7):743-755

Cho N, Chueh PJ, Kim C et al Monoclonal antibody to a cancer-specific and drug-responsive hydroquinone (NADH) oxidase from the sera of cancer patients. Cancer Immunology, Immunotherapy. 2002;51(3):121-9

Shapiro S, Beenhouwer DO, Feldmesser M et al. Immunoglobulin G Monoclonal Antibodies to Cryptococcus neoformans Protect Mice Deficient in Complement Component C3 Infect. Infection and immunity.2002;70(5):2598-604

Castro AR, Morrill We, Pope V. Lipid Removal from Human Serum Samples Clinical and diagnostic laboratory immunology.2000;7(2):197-199

Nussbaum G, Cleare W, Casadevall A et al Epitope Location in the Cryptococcus neoformans Capsule Is a Determinant of Antibody Efficacy The Journal of experimental medicine.1997;185:685-694


Nanoparticles

Human & Plasma Cell Lines.  Pan, Dipanjan, et al. "A strategy for combating melanoma with oncogenic c-Myc inhibitors and targeted nanotherapy." Nanomedicine 10.2 (2015): 241-251.

Plasma-Enzyme-Nanoparticle Mixture.  Pan, Dipanjan, et al. "Anti-Angiogenesis Therapy in the Vx2 Rabbit Cancer Model with a Lipase-cleavable Sn 2 Taxane Phospholipid Prodrug using αvβ3-Targeted Theranostic Nanoparticles." Theranostics 2014; 4(6):565-578


Microarray

Srinivasan, Harish."Antibody microarray as a proteomic tool for effective diagnosis and prediction of prognosis in cancer."(2014).


Saliva

Lucy E. DesJardin Isolation of M. tuberculosis RNA from Sputum Methods in Molecular Medicine.2001;48:133-139

Beenhouwer DO, Shapiro S, Feldmesser M et al. Both Th1 and Th2 Cytokines Affect the Ability of Monoclonal Antibodies To Protect Mice against Cryptococcus neoformans Infection and immunity.2001;69: 6445-6455

Desjardin LE, Perkins MD, Wolski K et al. Measurement of Sputum Mycobacterium tuberculosis Messenger RNA as a Surrogate for Response to Chemotherapy American journal of respiratory and critical care medicine.1999;160(1):203-10

Template Preparation Production Sequencing Protocols. Stanford Genome Technology Center


Patents

Shiffman, Dov, et al. " Methods for quantitation of insulin and c-peptide." U.S. Patent Application No. 15/942,188.

Iwakura Yoichiro, Kakuta Shigeru, Suzuki, Shunsuke - United States Patent Application 20130011413. Method and Pharmaceutical Composition for Treatment of Intestinal Disease

Mcintyre, John A. United States Patent: 20120107841. Serum Diagnostic Method, Biomarker and Kit for Early Detection and Staging of Alzheimer's Disease

Morre, James D et al. United States Patent: 20030170757. Monoclonal antibodies specific for neoplasia-specific NADH: disulfide reductase

DJ Morre, NM McCarty, D Morre et al United States Patent: 7053188. Monoclonal antibodies specific for neoplasia-specific NADH: disulfide reductase

David C. Jones. United States Patent: 7999084. Devices and methods for reducing matrix effects

J Krupey - United States Patent: 5885921 Hydrophobic silica adsorbents for lipids


Vaccine Research (Cholesterol Removal From Human Serum)

Kamtchoua, Thierry, Monica Bologa, Robert Hopfer, David Neveu, Branda Hu, Xiaohua Sheng, Nicolas Corde, Catherine Pouzet, Gloria Zimmerman, and Sanjay Gurunathan. Safety and immunogenicity of the pneumococcal pneumolysin derivative PlyD1 in a single-antigen protein vaccine candidate in adults.Vaccine (2012).