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NuGel™ PBA
제품명: NuGel™ PBA
용도: Glycoprotein Enrichment
메이커: Biotech Support Group
카달로그:


소개

 

NuGel™ Phenyl Boronic Acid

 

Polymer Coated Silica Affinity Matrices For Glycoprotein Enrichment


•  Non-specific sites are virtually eliminated by a polymer coating15. NuGel PBA.PNG

•  Stable across a wide pH range 4 – 10

•  1000Å, 50µm suitable for LC and batch processes

•  Cis-diol specific, enriches heterogeneous sets of glycoproteins

•  Unmasks glycoproteins from high abundance proteins, most notably albumin

•  Efficient new surface technology ideal for proteomic applications

•  Enriches glycoproteins from blood, serum, plasma, tissue or cell culture media.

•  Sorbitol elution at pH 7.5 – 8.5

•  Adaptable to liquid chromatography (LC) and high-throughput 96-well format


NuGel™ Phenyl Boronic Acid is a derivative of NuGel™ polyepoxy silica affinity

support. This affinity support contains phenyl boronic groups at the end of 

hydrophilic spacer arms and is used to bind ligands containing cis-diols, 

glycoproteins.


NuGel™ Phenyl Boronic Acid Technical Data

(Characteristics Of The Matrix)

•  Spacer Arm : Polymerized Hydrophilic Carbon Chain

•  Porosity : 1000Å

•  Average Particle Size : 50 um  


Special Features:

•  Binds ligands containing 1,2 cis-diol groups of glycoproteins.

•  pH stable from 4 to 10. 

 


☞   Click Here To View NuGel™ Phenyl Boronic Acid Product Sheet

사양

Glycoprotein Binding : approximately 5-10 mg/gm of NPBA matrix

주문정보

15. NuGel PBA_주문정보.PNG


15. NuGel PBA_주문정보2.PNG

관련자료

References


Patents

Monoclonal antibodies directed to the cytotoxic lymphocyte maturation factor European Patent EP0790255

Purification of immunoglobulins using affinity chromatography and peptide US 2006/0153834 A1


Affinity

Chaumet, Alexandre, Sandrine Castella, Laïla Gasmi, Aurélie Fradin, Gilles Clodic, Gérard Bolbach, Robert Poulhe, Philippe Denoulet, and Jean-Christophe Larcher. "Proteomic analysis of Interleukin enhancer binding factor 3 (Ilf3) and Nuclear Factor 90 (NF90) interactome." Biochimie (2013).

Dermot Walls, Robert McGrath and Sinéad T.Loughran A Digest of Protein Purification. Methods Molecular Biology. Volume 681: 3-23 (2011)

Ehrlich, G. K., Michel, H., Chokshi, H. P. and Malick, A. W. Affinity purification and characterization of an anti-PEG IgM. Journal of Molecular Recognition, 22: 99–103 (2009).

Development of hepatitis B virus capsids into a whole-chain protein antigen display platform: New particulate Lyme disease vaccines. International Journal of Medical Microbiology Volume 298, Issues 1-2, 3 January 2008, Pages 135-142

A sensitive and high-throughput assay to detect low-abundance proteins in serum Hongtao Zhang, Xin Cheng, Mark Richter & Mark I Greene. Nature Medicine 12, 473 - 477 (2006)

Transformation of a L-peptide epitope into a D-peptide analog. Peptides Frontiers of Peptide Science American Peptide Symposia, 2002, Volume 5, Session XI, 769-770

Expression and folding of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm. Research in Microbiology Volume 153, Issue 7, September 2002, Pages 469-474

Identification of model peptides as affinity ligands for the purification of humanized monoclonal antibodies by means of phage display Journal of Biochemical and Biophysical Methods Volume 49, Issues 1-3.2001

George K. Ehrlich, Pascal Bailon, Wolfgang Berthold. Phage Display Technology - Identification of Peptides as Model Ligands for Affinity Chromatography Affinity Chromatography Methods in Molecular Biology, 2000, Volume 147, 209-220

A Digest of Protein Purification and partial amino acid sequence of a 28 kDa cyclophilin-like component of the rat liver sigma receptor. Life Sciences , Volume 55, Issue 8, 1994.

Nachman, M., Azad, A. R. M. and Bailon, P. (1992), Efficient recovery of recombinant proteins using membrane-based immunoaffinity chromatography (MIC). Biotechnology and Bioengineering, 40: 564–571.

Kinetic aspects of membrane-based immunoaffinity chromatography. Journal of Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages 167-172

Identification of model peptides as affinity ligands for the purification of humanized monoclonal antibodies by means of phage display. Methods in Molecular Biology, 2000, Volume 147, 209-220

Membrane-based receptor affinity chromatography. Journal of Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages 155-166 9th International Symposium on Affinity Chromatography and Biological Recognition


Ion Exchange

Levin W Protein Purification of recombinant human secretory phospholipase A2 (group II) produced in long-term immobilized cell cultureExpr Purif 1992 Feb;3(1):27-35.