Product
소개
HemogloBind™ Hemoglobin Depletion From Hemolyzed Serum/Plasma
• Has a high degree of specificity for hemoglobin binding up to 10 mg/ml
• Applications in blood substitutes, enzyme recovery and analytical interferences
• Removes hemoglobin from any species including human, sheep, bovine, goat,
etc
• Removes hemoglobin from organs, tissues.
• Hemoglobin removal from red blood cell lysate for proteomics and
biomarker drug discovery
• The flow through fractions retain their enzymatic and biological activity
• The flow through fractions are compatible with LC-MS, activity based protein
profiling and proteomic studies.
HemogloBind™ comes from a class of solid-phase, or surface-based, elastomeric poly-electrolytic surfaces that bind proteins through an empirically derived chemistry combining elements of polymer composition, cross-linking architecture and charge properties. HemogloBind™ is engineered for a high degree of selectivity and does not cross react with most common serum components, making it an excellent tool in numerous applications. These include analytical protocols where optical interference is problematic, such as bilirubin analysis and bulk serum clarification. Hemoglobin variants, as in thalassemia, bind with differential affinity towards HemogloBind™. For purification and/or analysis of hemoglobin, a modest elevation in pH will facilitate desorption of hemoglobin bound to HemogloBind™
사양
주문정보
관련자료
References
Hemolyzed Serum
Craig, J. R., et al. "A comparison of the anatomical and gastrointestinal functional development between gilt and sow progeny around birth and weaning." Journal of animal science (2019).
Reticulocytes
Nguyen, Anthony T., et al. "UBE2O remodels the proteome during terminal erythroid differentiation." Science357.6350 (2017): eaan0218.
Hemocyte fractions
O’Connell, Grant C., et al. "Monocyte-lymphocyte cross-communication via soluble CD163 directly links innate immune system activation and adaptive immune system suppression following ischemic stroke." Scientific reports 7.1 (2017): 12940
Perfusates
Laing, Richard W., et al. "The use of an acellular oxygen carrier in a human liver model of normothermic machine perfusion." Transplantation 101.11 (2017): 2746.
Hemolysate
Dasauni, Pushpanjali, et al. "Refractive index of blood is a potential qualitative indicator of hemoglobin disorder in humans." Journal of Proteins & Proteomics 9.3 (2018).
Cell lysates of reticulocytes
Junqueira, Caroline, et al. "Cytotoxic CD8+ T cells recognize and kill Plasmodium vivax–infected reticulocytes." Nature medicine (2018): 1.
Whole blood aliquots
Pácal, Lukáš, et al. " Deleterious Effect of Advanced CKD on Glyoxalase System Activity not Limited to Diabetes Aetiology ." International Journal of Molecular Sciences 19.5 (2018): 1517.
Total hemocyte lysates
O'Connell, Grant C., et al. "Monocyte-Lymphocyte Cross-Communication Via Soluble CD163 Directly Links Innate Immune System Activation And Adaptive Immune System Suppression Following Ischemic Stroke
Dried Blood Spot(DBS)/Whole Blood
Asleh, Rabea, et al. " Haptoglobin Genotype-dependent Differences in Macrophage Lysosomal Oxidative Injury." Journal of Biological Chemistry289.23 (2014): 16313-16325.
Biological Fluids
J Krupey - United States Patent: 10/180,053, 2002 Removal of extraneous substances from biological fluids containing nucleic acids and the recovery of nucleic acids