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HemoVoid™ Blood Card Kit
제품명: HemoVoid™ Blood Card Kit
용도: Hemoglobin Removal Kits
메이커: Biotech Support Group
카달로그:


소개

 

HemoVoid™ Blood Card Kit      Hemoglobin Depletion And Protein Enrichment From Dried Whole Blood Cards

 

Substantially reduces hemoglobin interference 10, HemoVoid™ Blood Card Kit.png

from dried blood spot protein analytes


•  Hemoglobin voids in flow-through >98%, with <30 minute bind/wash/elute 

   protocol

•  Hemoglobin removal from whole blood lysates extracted from dried blood 

   cards

•  Hemoglobin removal from frozen and fresh whole blood•Blood proteins 

   and enzymes areenriched for potential biomarker and proteomic studies.

•  Disposable, cost-effective hemoglobin depletion sample preparation protocol

•  Removes hemoglobin from whole blood of diverse species including human,

   mice, sheep, bovine, goat, rat, etc.

 

Hemoglobin is a common contaminant from whole blood. The HemoVoid™ 

Blood Card protocol was designed to substantially reduce the presence of 

hemoglobin and its associated interference with many serum protein analytes. 


HemoVoid™ Blood Card is a silica based polyelectrolyte matrix, removes hemoglobin from dried whole blood card samples. 

The HemoVoid™ protocol uses mild buffers; the protocol conditions are so gentle that native enzyme activity is retained in elution fractions.

 


Hemovoid Blood Card Research Applications.png

 


The HemoVoid™ Blood Card produces enriched proteins free of hemoglobin which are used for biomarkers and protein analysis (B). The hemoglobin enriched filtrate could have hemoglobin variants, hemoglobin binding proteins or other analytes optimal for biomarker studies (A).  

 

 

☞   Click Here To View HemoVoid™ Blood Card 

 

사양

10, HemoVoid™ Blood Card Kit_사양.png

주문정보

10, HemoVoid™ Blood Card Kit_주문정보.png

 

10, HemoVoid™ Blood Card Kit_주문정보2.png

관련자료

References 

 

Human Red Blood Cells (RBC)

HemoVoid™ On Bead Digestion Application Work On RBC by Irene Granlund, Umeå University 

Red Blood Cells, Plasmodium extracts

Machado, Patrícia Isabel Pires. Pyruvate kinase and glucose-6-phosphate dehydrogenase deficiencies and their association with malaria–population genetics and proteomic studies. Diss. Universidade do Porto, 2013.

Walpurgis, Katja, et al. "Effects of gamma irradiation and 15 days of subsequent ex vivo storage on the cytosolic red blood cell proteome analyzed by 2D DIGE and Orbitrap MS." PROTEOMICS-Clinical Applications (2013).

P. Falciparum Clone 3D7 Cultured In Human Erythrocytes

Lasonder E, Green JL, Camarda G, Talabani H, Holder AA, Langsley G, Alano P. The Plasmodium falciparum schizont phospho-proteome reveals extensive phosphatidylinositol and cAMP-Protein Kinase A signalling. J Proteome Research. 2012;

Red Blood Cell Lysate

Barasa, Benjamin, and Monique Slijper. "Challenges for red blood cell biomarker discovery through proteomics." Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1844.5 (2014): 1003-1010.

Lange, Philipp F., Pitter F. Huesgen, Karen Nguyen, and Christopher M. Overall. "Annotating N termini for the Human Proteome Project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome." Journal of proteome research (2014).

Katja Walpurgis, Maxie Kohler, Andreas Thomas et al.Validated hemoglobin-depletion approach for red blood cell lysate proteome analysis by means of 2D-PAGE and Orbitrap MS.Electrophoresis.2012;

Mizukawa, B., George, A., Pushkaran, S. et al. Cooperating G6PD mutations associated with severe neonatal hyperbilirubinemia and cholestasis.Pediatric Blood Cancer.2011;56: 840-842.

Sudha Neelam, David G Kakhniashvili, Stephan Wilkens et al. Functional 20S proteasomes in mature human red blood cells Experimental Biology and Medicine.2011;236:580-591