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NuGel™ PBA Kit
제품명: NuGel™ PBA Kit
용도: Glycoprotein Enrichment
메이커: Biotech Support Group
카달로그:


소개

 

NuGel™ PBA Kit     With Phenyl Boronic Acid NuGel™ Matrix

 

Glycoprotein Enrichment PBA Kit15. NuGel PBA.PNG


•  Cis-diol specific, enriches heterogeneous sets of glycoproteins

•  Unmasks glycoproteins from high abundance proteins, most notably albumin

•  Disposable, no column regeneration or cross-contamination

•  Efficient new surface technology ideal for proteomic applications

•  Enriches and purifies glycoproteins from blood, serum, plasma, tissue or cell

   culture media.

•  Removes > 90% of serum albumin (the non-glycosylated fraction)

•  Sorbitol elution at pH 7.5 – 8.5

•  Adaptable to a high-throughput 96-well format 

 

Silica has been an industry standard as an advantageous matrix suitable for 

high performance liquid chromatography. With NuGel™, non-specific sites 

have been virtually eliminated making it an ideal support for affinity 

purification. Through a proprietary polymer coating, silica is crosslinked 

forming a reactive Poly-Epoxy functionality stable across a wide pH range 

(pH 2 to 10). From this foundational chemistry, all of the NuGel™ affinity 

products are derived.


The Phenyl Boronic Acid (PBA) ligand is immobilized through the NuGel™ poly-Epoxy linkage with attachment through the amino group. While various lectins bind to specific saccharide residues, the PBA ligand binds to the 1,2-cis-diol groups of biomolecules and enriches for heterogeneous sets of glycoproteins containing both N-linked and O-linked oligosaccharides. An easy and fast spin-filter format makes glycoprotein enrichment simple starting from 50µl serum, or 1-2 mg total protein.

 


☞  Click Here For NuGel™ Glycoprotein Enrichment PBA Kit Product Sheet

사양

16. NuGel PBA Kit_사양.PNG

주문정보

16. NuGel PBA Kit_주문정보.PNG

 

16. NuGel PBA Kit_주문정보2.PNG

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