KinaSorb™    

Kinase (& ATP binding proteins) enrichment reagent

•  Non-covalent immobilization of phosphate group with optimal nucleotide 

   orientation & specificity

•  Enrichment 3-5X Kinase enrichment, protein recoverable, ~200 µg

•  60 minute, scaleable protocol compatible with functional assays, 

   electrophoresis and LC-MS

•  Phosphatase activity & cyclic nucleotide phosphodiesterase (PDE) activity not 

   detectable

•  Improves protein normalization when comparing heterogeneous tissues

•  On-bead digestion protocols for LC-MS annotation 

KinaSorb™ is a new reagent kit used for the enrichment and isolation of kinases. ATP – the common substrate for kinases, is reversibly immobilized with optimal orientation and specificity, to metallic oxide particles, producing a single-use highly efficient enrichment method for kinase & other ATP binding proteins. 

The standard prep protocol starts with 100 µl of clarified cellular extracts, or approximately 2 mg total cellular extract protein, but the process can be scaled up or down to accommodate different sample volumes and protein concentrations. 

The kit includes all necessary reagents for immediate use.