Apo Active 3 FITC

 

Antibody Specific Active Caspase 3 Detection Kit

 

 

1.  Key Benefits

 

•   Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents

    that tend to cross-react with caspase 7 and other caspases

•   Applications – Works with a fluoresence microscope, 96 well plate reader, or flow cytometer 

•   Yields both quantitative and qualitative results. Gives a strong positive signal 

•   Can be used in conjunction with other antibodies or stains 

•   No need to make cell lysates or run western blots. Cells can be fixed and analyzed later 

•   Works with human, mouse and rat cell lines

 

 

2.  Assay Principle

 

Cell Technology ’s APO-ACTIVE CASPASE 3 Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary FITC labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal.

 

 

 

 

Fig (A). Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.

 

 

Fig (B). Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with FITC labeled Goat anti Rabbit antibody.

Reagent	Part#	Temperature
Rabbit Anti Cleaved Caspase 3 Affinity Purified Polyclonal Antibody, 1 Vial	Part # 1002	2-8°C
FITC Labeled Goat Anti Rabbit Affinity Purified Polyclonal Antibody, 1 Vial	Part # 2002	2-8°C
10X Fixative, 1 Vial	Part # 3001	2-8°C

 

Product Data Sheet        Protocol        MSDS

 

Reference

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Cytotoxicity and Secretion of Gamma Interferon Are Carried Out by Distinct CD8 T Cells during Mycobacterium tuberculosis Infection - Thorbjorg Einarsdottir, Euan Lockhart, and JoAnne L. Flynn - American Society for Microbiology - Infection and Immunity October 2009, p. 4621-4630, Vol. 77, No. 10.

Converting cell lines representing hematological malignancies from glucocorticoid-resistant to glucocorticoid-sensitive: Signaling pathway interactions - Anna S. Garza, Aaron L. Miller, Betty H. Johnson, E. Brad Thompson - Leukemia Research Vol Vol 33, Issue 5, pp 717-727. May 2009