Fluoro ATP

 

Fluorescent ATP Detection Kit - Detection of ATP in tissue extracts/cell samples

 

 

Adenosine Triphosphate (ATP) is an organic molecule which is exclusively formed in the mitochondria. Vritually all living systems store energy in the phosphate bonds of ATP and plays a centralrole in cellular metabolism and energy transfer reactions. ATP and be de-phosphorylation to ADP and further de-phosphorylation to AMP. This cycling of ATP is central to transferring potential (thermodynamic) energy from one source to another. There are several genetic disorders that effect the generation of ATP in the mitochondria 

 

 

1.  Key Benefits 

 

• Detection of ATP in cells or tissue extracts.

• Detection of ATP in cell death, energy metabolism, mitochondria function

• ATP measurement in ATP consuming enzymes such as Kinases and ATPases

• ATP detection in Bacterial, Fungal and Plant Cells

• Fluorescent 96 well Plate Reader Readout (excitation: 530-570nm and emission at 590-600nm).

 

  

2.  Assay Principle

 

The Fluoro ATP detection kit utilizes a non-fluorescent detection reagent, which is reduced in the presence ATP and a coupled enzyme reaction to produce its fluorescent analog. There is a linear relationship of ATP concentration to the fluorescent analog concentration. An ATP standard curve is generated to interpolate sample ATP concentrations. The kit can be used in both endpoint and kenitic modes.

 

Reaction:

1.  ATP + Enzyme Coupled Reaction + Non-Fluorescent Detection Reagent→Fluorescent Analog + AMP

    Detection: Excitation at 530-570nm and Emission at 590-600nm

2.  50µL of sample or ATP Standard+ 50µL of Enzyme Reaction Cocktail -> Incubate 30 – 60 minutes; RT; DARK

    Detection: Excitation at 530-570nm and Emission at 590-600nm

 

ATP Spike	% Recovery	% Recovery
	no NEM	40mM NEM
25.5	170	95.85
8.35	230	102
2.154	227	86.75

Table 1. ATP was spiked into Jurkat cell samples in Substrate Buffer (Part# 3046) with or without 40mM NEM (Part# 7026). % Recovery was determined via linear regression from ATP standard curve. N=3 per sample.

Fluoro ATP	FLATP100-2	100 tests
Fluoro ATP	FLATP100-3	500 tests

Fluoro ATP Protocol

 

Fluoro ATP Datasheet

 

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