Fluoro Phosphate

  

Phosphate Detection Kit (Fluorescence)

 

Phosphorus is essential for multiple and diverse biological functions, including cellular signal transduction, mineral meatbolism, and energy exchange. Elevated levels of serum phosphate may be associated with a higher risk of death and adverse cardiovascular outcomes in people with prior myocardial infarction, and is the subject of large amount of research.

 

 

1.  Key Benefits 

•  Quantitation of phosphate anion in blood, plasma and serum samples.

•  Sensitive – can detect upto 200nM phosphate

•  Easy to use – Fluorescence (ex/em 535/585nm or Absorbance readout @ 570nm)

 

2.  Assay Principle

Cell Technology’s Phosphate detection kit provides a simple, one-step fluorimetric or colorimetric method for determination of phosphate in serum and plasma samples. The assay is based on an enzyme-coupled reaction that detects inorganic phosphate. The substrate is converted to Hydrogen peroxide in presence of inorganic phosphate in a coupled enzymatic reaction. The Hydrogen Peroxide then reacts with the detection reagent in a 1:1 stoichiometry in presence of Horse Radish Peroxidase to produce the stable fluorescent product.

 

Substrate + Pi

 

↓ Coupled enzyme reaction

 

H2O2

 

↓ Horse Radish Peroxidase + Detection Reagent

 

Fluorescent Product

 

λmax 570nm. λ ex/em 535/585nm.

 

Colorimetric assay can be read on a spectrophotometer at 570nm.

 

Fluorescence is measured at excitation 530nm and emission 585nm. 

Reagent	Catalog #	Size
Fluoro Phosphate Detection Kit	FLPHOS100-2	100 tests

 

Fluoro Phosphate Protocol

 

Fluoro Phosphate Datasheet

 

msds.fluoroPhos