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Fluoro NAD
제품명: Fluoro NAD
용도: NAD/NADH Detection Kit (Fluorescence)
메이커: Cell Technology
카달로그:


소개

 

Fluoro NAD

  

The role of pyridine nucleotides (NAD/NADH) metabolism in health, is of continual and increased interest. A growing amount of evidence supports the fact that NAD metabolism regulates important biological effect including life span. NAD, through poly-ADP-ribosyl polymerase (PARP), mono-ADP-ribosyltransferase (ARTs) and recently characterized sirtuin enzymes, exerts potential biological effects. These enzymes modify proteins to regulate their function via ADP-ribosylation or deacetylation and are involved in several pathways including apoptosis, DNA repair, senescence and endocrine signaling. This suggests that either the enzymes or NAD could be an important therapeutical target 1.

 


1.  Key Benefits 


•  Detection of NAD/NADH content in cells or tissue extracts.

•  Detection of NAD/NADH levels in apoptosis, metabolism, proliferation, DNA repair, senescence, endocrine signaling and life span.

•  NAD/NADH detection in Bacterial, fungal and plant cells.

•  Fluorescent 96 well Plate Reader Readout (excitation: 530-570nm and emission at 590-600nm). 

 

 

2.  Assay Principle


The Fluoro NAD/NADH detection kit utilizes a non-fluorescent detection reagent, which is reduced in the presence NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction. The enzyme reaction specifically reacts with NAD/NADH and not with NADP/NADPH

 

Reaction :

1. NADH + non-fluorescent detection reagent+ electron→coupler fluorescent analog + NAD

2. NAD + enzyme coupled reaction→ NADH (then proceeds to reaction 1).

 

excitation: 530-570nm and emission at 590-600nm

 

 

Fluorescent_NAD_NADH-300x218.png



Figure 1. NADH standard curve titrated in NAD/NADH lysis buffer. Incubation time = 1 hour.

  


Fluorescent_NADH_Assay-300x193.png

  

Figure 2. NAD/NADH Assay showing no cross-reactivity with NADPH. Incubation time = 1 hour. 

 

 

주문정보

Reagent

Catalog #

Size

Fluoro NAD/NADH Detection Kit

FLNADH100-2

100 tests  

Fluoro NAD/NADH Detection Kit

FLNADH100-3

500 tests

 

 


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