Fluoro Cholesterol

  

Cholesterol Assay Kit (Fluorescence/Colorimetric)

 

Cholesterol is a lipid present in the cell membranes of eukaryotes and circulates in the blood stream. It is used in the biosynthesis of hormones, and plays an important role in cell signaling processes. Cholesterol exists as a free acid, as well as, in the esterified form as cholesteryl esters. Elevated levels of cholesterol are indicated in atherosclerosis and heart disease, and are the subject of large amount of research focused on cholesterol metabolism. Quantitative determination of cholesterol in experimental samples is central to this research.

 

Cell Technology’s Total Cholesterol kit is a simple one step assay which can be used either as a Fluorimetric or Colorimetric assay to help detect the level of total cholesterol in samples. 

 

 

1.  Key Benefits 

 

  •  Detection of Total Cholesterol in Biological Samples.

  •  Easy to Use -Simple ONE STEP assay.

  •  Used to study the effect of drugs on Cholesterol Metabolism.

  •  Highly Sensitive – Detects up to 200nM of Cholesterol.

  •  Versatile – Fluorimetric or Colorimetric readout in a 96 well format.

 

2.  Assay Principle

Cell Technology’s Cholesterol assay kit provides a simple, one-step fluorimetric or colorimetric method for the determination of total cholesterol in serum and plasma samples. The assay is based on an enzyme-coupled reaction that detects both free cholesterol and cholesterol esters. Cholesterol esters are hydrolyzed by cholesterol esterase into cholesterol, which is then oxidized by cholesterol oxidase to yield hydrogen peroxide and cholest-4-en-3-one (ketone). The hydrogen peroxide then reacts with the cholesterol probe (detection reagent) in a 1:1 stoichiometry to produce the stable fluorescent product

Cholesterol ester

↓ Cholesterol esterase

Cholesterol

↓ Cholesterol Oxidase

H2O2 + Cholest-4-en-3-one

↓

Cholesterol probe

 

Fluorescent Product

 

λmax 570nm. λ ex/em 535/585nm.

 

Colorimetric assay can be read on a spectrophotometer at 570nm.

 

Fluorescence is measured at excitation 530nm and emission 585nm.

 

Reagent	Catalog #	Size
Fluorescent Total Cholesterol Detection Kit	FLCHOL100-2	100 tests

 

 

Total Cholesterol Protocol

 

Total Cholesterol Datasheet

 

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