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Fluoro NADP
제품명: Fluoro NADP
용도: NADP/NADPH Detection Kit (Fluorescence)
메이커: Cell Technology
카달로그:


소개

 

Fluoro NADP

  

NADP/NADPH Detection Kit (Fluorescence)

 

Nicotinamide adenine dinucleotide phosphate (NADP+) is used in anabolic reactions, such as lipid and nucleic acid synthesis, which require NADPH as a reducing agent. NADPH is the reduced form of NADP+, and NADP+ is the oxidized form of NADPH. In cells, NADPH plays the role of a carrier of reducing power and is primarily involved in maintaining optimal redox metabolism. A simplified assay for the measurement of NAD and NADP is critical to understanding the roles of these pyridine nucleotides in normal and abnormal cells.

 

NADPH is produced in the oxidative phase of the pentose phosphate pathway in cells, a multifunctional pathway whose primary purpose is to generate reducing power in the form of NADPH. NADPH is a cofactor for enzymes that synthesize energy-rich molecules and provide the reducing equivalents for the oxidation-reduction involved in protecting the cell from the toxicity of reactive oxygen species (ROS) and NADPH oxidase-dependent ROS generation. Both NAD and NADP have been shown to influence hemoglobin affinity for oxygen in erythrocytes. In plant cells, NADPH is used as the reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis (1-2).

 

Cell Technology’s Fluoro NADP/NADPH provides a highly reliable, sensitive fluorometric assay for the quantification of NADP, NADPH and their ratio in biological samples. 

 

 

1.  Key Benefits 


•  Detection of NADP/NADPH content in cells or tissue extracts.

•  Detection of NADP/NADPH levels in antioxidation and oxidative stress.

•  Detection of NADP/NADPH levels in cell death, energy metabolism, and mitochondria function.

•  Species Independent – NADP/NADPH detection in Bacterial, fungal and plant cells.

•  Highly Sensitive – Detects up to 4nM NADP and NADPH.

•  Highly Specific – No Cross reactivity with NAD/NADH.

•  Easy to Use – 96 well Fluorescent Plate reader readout.

 


2.  Assay Principle


The Fluoro NADP/NADPH detection kit utilizes a non-fluorescent detection reagent, which is reduced in the presence NADPH to produce its fluorescent analog and NADP. NADP is further converted to NADPH via an enzyme-coupled reaction. The enzyme reaction specifically reacts with NADP/NADPH and not with NAD/NADH.

 

 

Fluorescent_NADP_NADPH_1-300x293.png


Figure 1. Comparison of NADPH & NADH Std Curves

 

 

Fluorescent_NADP_NADPH_2-300x237.png

 

 

Figure 2. Comparison of NADP & NADPH Std Curves 

 


주문정보

Reagent

  Catalog #

Size

Fluorescent NADP/NADPH Detection Kit

  NADPH100-2

100 tests  

Fluorescent NADP/NADPH Detection Kit

  NADPH100-3

500 tests

 


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