Fluoro Hydroxyl/ Peroxynitrite (Cell Permeable)

Hydroxyl (OH)/ Peroxynitrite (ONOO-) Detection Kit

A new novel probe, Hydroxyphenyl fluorescein (HPF), developed by Tetsuo Nagano et. al. (1), is a highly selective probe for the detection of highly Reactive Oxygen Species (hROS). It is a cell permeable highly sensitive fluorescent probe for hydroxyl radical (OH•), and peroxynitrite (ONOO-) detection. It has little reactivity towards other hROS such as: hypochlorite (-OCl), singlet oxygen (021), superoxide (02-•), hydrogen peroxide (H202), nitric oxide (NO•), and alkyl peroxide (RO2•) (see table below)1.

Key Benefits 

•  Quenched Cell permeable dye.

•  One Step, No wash Homogenous assay.

•  Adaptable to High throughput assay platforms.

•  Can monitor multiple time points to follow real time kinetics.

•  Non-destructive cell based assay allows monitoring of additional parameters.

•  Applications – Fluorescent plate reader / Flow Cytometery / Fluorescent Microscopy.

HPFProtocol

Fluoro HPF Datasheet

msds.APF-HPF-OCl