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aCella™ SEAP
제품명: aCella™ SEAP
용도: Secreted Alkaline Phosphatase (SEAP) Reporter Gene Assay Kit (Chemiluminescent)
메이커: Cell Technology
카달로그:


소개

 

aCella™ SEAP

  

Secreted Alkaline Phosphatase (SEAP) Reporter Gene Assay Kit (Chemiluminescent)

 

 

1.  Key Benefits 

•  Highly effective and stable chemiluminescent assay for SEAP.

•  Extremely sensitive, detect alkaline phosphatase at attogram level.

•  Simple and fast assay-add the reagent directly to your experimental samples. Plate can be incubated and read in 5 min.

•  Works for cell culture media and tissue lysates.

 


2.  Assay Principle

Secreted alkaline phosphatase (SEAP) is widely used as a reporter to study gene expression. SEAP is secreted into the culture supernatant of the transfected cell lines. SEAP activity in the culture medium reflects changes in intracellular concentrations of SEAP mRNA and protein. The changes of gene expression can be easily assayed using the same cultures by repeated sampling. Cell Technology’s aCellaTM – SEAP detection kit utilizes a Chemiluminescent-based assay technique to detect SEAP reporter gene expression. It provides an extremely sensitive tool to quantitate SEAP and analyze transcriptional and promoter activity in transfected cells.

 

In the assay, a chemiluminescent substrate was used to detect the Secreted Alkaline Phosphatase in cell culture. The stabilized 1, 2-dioxetane substrate provide high signal to noise ratio, wide dynamic range, rapid results and excellent reproducibility. The SEAP enzyme transfers the phosphoryl residue via a phosphoryl-enzyme intermediate. The intermediate is not stable and decomposed and emits light for detection. The assay can detect alkaline phosphatase at attogram level. The kit provides sample material for 100 assays in a 96-well plate format.


주문정보

aCella™ SEAP - 100

  5063

100 tests  

 


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