Fluoro hROS (Cell Permeable)

hROS Detection Kit (Fluorescence)

A new novel probe, Aminophenyl fluorescein (APF), developed by Tetsuo Nagano et. al. (1), is a general selective indcator for the detection of highly Reactive Oxygen Species (hROS). The probe has little reactivity towards other ROS such as: singlet oxygen (O21), superoxide (O2– •), hydrogen peroxide (H2O2), nitric oxide (NO•), and alkyl peroxide (RO2•) (see table below)1. APF is a cell permeable indicator that can be used to detect Hydroxyl Radical (.OH), Peroxynitrite: (ONOO- ) and hypochlorite (-OCl) production in cells.

Several fluorescent probes such as 2’,7’-dichlorodihydrofluorescein (DCFH) and di-hydrorhodamine123, have been developed to detect Reactive Oxygen Species (ROS). As Hempel and co-workers (2) have indicated, DCFH and di-hydrorhodamine123 can react with various ROS and oxidizing species such as hydrogen peroxide, hypochlorite, superoxide, nitric oxide, ferrous ion, and others. These probes should be considered as detecting a broad range of ROS (2).

Key Benefits 

•  Quenched Cell permeable dye.

•  One Step, No wash Homogenous assay.

•  Adaptable to High throughput assay platforms.

•  Can monitor multiple time points to follow real time kinetics.

•  Non-destructive cell based assay allows monitoring of additional parameters.

•  Applications – Fluorescent plate reader / Flow Cytometery / Fluorescent Microscopy.

hROSProtocol

Fluoro hROS Datasheet

msds.APF-HPF-OCl