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aCella™ TOX
제품명: aCella™ TOX
용도: Non Radioactive Cytotoxicity Assay (Bioluminescence)
메이커: Cell Technology
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소개

 

aCella™ TOX

  

Non Radioactive Cytotoxicity Assay (Bioluminescence)

 

 

1. Key Benefits 

 

•  Safe – Non Radioactive Enzyme release assay.

•  Versatile – Useful for measuring activity of T Cells, Primary Cells, NK, complement and other lytic agents.

•  Assay can be run in serum supplemented media.

•  Homogenous – One-step, no wash assay. Assay can be run in same plate as samples.

•  FAST – Results in 3-5 minutes. Chromium 51 or europium release for measurement are time consuming. The inherent sensitivity

   of luciferase detection is enhanced by the amplification effect of enzyme turnover, which produces thousands, millions or

   billions of high – energy molecules for each molecule of the enzyme.

•  Highly Sensitive – Can detect fewer than 500 cells/well in the presence of serum or as few as 10 cells/well in serum-free or

   heat-killed media.

•  GAPDH: The fact that GAPDH is a natural component of cells, and does not need to be introduced into the cells in any manner,

   distinguishes this assay from all methods which require prelabelling of cells, transfection, transformation, or other methods of

   introducing proteins or other molecules into the target cells in order to generate a signal in a later step.

•  Advantages for measurement of cell mediated or complement mediated cytolysis – It is usually desirable to use smaller numbers

   of T Cells than are needed for the 51Cr – release assay, since excessive numbers of effector cells can increase the background

   signal. This is now possible due to the high sensitivity of aCella-Tox.

•  ADCC / CMC Assays – A non radioactive alternative to 51Cr assays. Please click here for a direct comparison between the 

   aCella-TOX and (51Cr) Chromium Release Methods

•  HTS – Adaptable for High Throughput format

•  Non-destructive assay allows monitoring of additional parameters.

 


2.  Assay Principle

 

GAPDH is an important enzyme in the glycolysis and gluconeogenesis pathways. This homotetrameric enzyme catalyzes the oxidative phosphorylation of D-glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate in the presence of cofactor and inorganic phosphate. In the aCella-TOX reaction scheme the release of GAPDH is coupled to the activity of the enzyme 3-Phosphoglyceric Phosphokinase (PGK) to produce ATP. ATP is detected via the luciferase, luciferin Bioluminescence methodology. 

 

Further, aCella-TOX is a homogeneous cytotoxicity assay; alternatively in dual mode, aCella-TOX can measure cytotoxicity and cell viability in the same plate. Culture supernatants can also be removed from the original plate and assayed in a different plate, allowing kinetics runs to be set up. The assay is non-destructive, allowing the monitoring of additional parameters such as gene expression.

 

주문정보

aCella - TOX

  CLATOX100-3

500 tests  

aCella - TOX (w/ 5 Lumi Plates)

  CLATOX100-3L

500 tests

aCella - TOX (w/ 5 Lumi Plates + 5 Tissue Culture Plates)

  CLATOX100-3P

500 tests  

aCella - TOX

  CLATOX100-4

1000 tests

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