인성크로마텍 로고

검색버튼
퀵메뉴 펼침

o
p
e
n
c
l
o
s
e

aCella™ AChE
제품명: aCella™ AChE
용도: Non Radioactive Assay for monitoring Acetylcholinesterase activity (Bioluminescence)
메이커: Cell Technology
카달로그:


소개

 

aCella™ AChE

  

Non Radioactive Assay for monitoring Acetylcholinesterase activity (Bioluminescence)

 


1.  Key Benefits 

 

•  Safe – Non Radioactive Enzyme activity assay.

•  Versatile: Nerve Gas, Pesticide Monitoring, drug screening Applications

•  Homogenous – One-step, no wash assay.

•  FAST – Results in 30 secs – 5 minutes.

•  Highly Sensitive

•  HTS – Adaptable for High Throughput format

•  Applications – Standard luminometer readout

 


2.  Assay Principle

 

We have developed a highly sensitive, very rapid, extremely simple assay for AChE activity, using the natural substrate, acetylcholine. As shown in Figure 1, a series of coupled enzyme reactions quickly translates the presence of active AChE into a change in the luminance of the reaction. First (reaction I), acetylcholine is hydrolyzed by the AChE to yield acetate and choline. The acetate and choline then enter a coupled enzyme reaction (reaction II) that results in consumption of ATP, and finally the ATP concentration is measured by the well-established luciferase method (reaction III). These reactions can occur simultaneously, and the result is generally obtained in five minutes or less. Inhibitors of AChE are readily detected by an increase in luminance due to reduced consumption of ATP.

 

The following reaction illustrates the sequence of events if AChE inhibitors are present:


Reaction I:    AChE + Inhibitor -> No Acetate and Choline

Reaction II:   Coupled Enzyme Reaction + ATP -> Reaction does not proceed

Reaction III:  ATP (remaining) + Luciferase/Luciferin -> LIGHT

 

 

aCellatm_AChE2-A-350x250.png


Figure A. Tacrine (a mixed-mode inhibitor of AChE) was serially diluted in DI water. Next 10mL of the diluted Tacrine (x axis labeling represents mM final concentration of Tacrine) was added to a white opaque 96-well microplate along with 50 mL of component A (AChE enzyme). The samples were incubated for 5 minutes after which 50mL of component B was added to all the wells. Data were collected using a luminometer. Data shown represents T=2 minutes after the addition of component B.

 

 


aCellatm_AChE1-B-300x220.png

 

 

Figure B. Malathion, a common pesticide, was first diluted in DMSO and subsequently serially diluted in Di water. 10μL of the diluted Malathion (x axis represents μM final concentration of Malathion) was added to a white opaque 96-well microplate followed by 50 μL of component A (AChE enzyme). The mixture was incubated for 15 minutes, after which 50 μL of component B was added to all the wells. Data were collected using a luminometer. Data shown is at T= 5 minutes. Data shown represents T=2.5 minutes after the addition of component B. 


주문정보

aCella - AChE

  CLACHE100-2

100 tests  

aCella - AChE

  CLACHE100-2

500 tests

aCella - AChE

  CLACHE100-2

1000 tests  

관련자료