Adeno-Associated Virus Purif. Mini Kit

 

 

Purification of recombinant AAV2 and AAV-DJ from rAAV vector transfected cell line

 

 

Adeno-assiciated viruses, belong to the replication deficient parvovirus family, 

are small single-stranded DNA viruses. AAVs are important gene delivery tools, 

which have been used in gene therapy and RNAi delivery.

Traditionally AAVs are purified by ultracentrifugation using CsCl to separate the 

virus particles from cellular proteins and media components.

The CsCl ultracentrifugation procedure is time consuming and limited to the volume 

of cell lysate to be processed.

The ViraTrapTM AAV mini purification kit is designed for efficient purification of recombinant AAV2 and AAV-DJ from rAAV vector transfected cell line. 

Up to 1 x 10^12 viral particles can be purified from cell lysate of 2 x T75 flasks. Each column can be regenerated for purifying the same rAAV. 

For optimized viral binding and recovery, each column can be regenerated only once. 

 

 

V1169-00           Adeno-Associated Virus Purification Mini Kit, serotype 2 and DJ           2 Prep

V1169-01           Adeno-Associated Virus Purification Mini Kit, serotype 2 and DJ           10 Prep

V1169-02           Adeno-Associated Virus Purification Mini Kit, serotype 2 and DJ           20 Prep