Plasmid Midi Kit I

 

 

 

 

Key to this kit is our proprietary DNA binding systems that allow DNA exclusively and 

efficiently bind to our ezBindTM matrix while proteins and other impurities are 

removed by wash buffer. Nucleic acids are easily eluted with sterile water or Elution Buffer.

 

Unlike other kits in the markets, our patented plasmid purification kit has no chaotropic 

salts in the buffers, the purified DNA is guanidine/anion exchange resin residues free which 

enable the high performance of downstream applications.

 

This kit is designed for fast and efficient purification of plasmid DNA from 15 to 50 mL of E. coli culture. 

The midi column has a plasmid DNA binding capacity of 250 µg. The yield from 50 mL culture is typically around 150 to 250 µg.

The purified DNA is ready for high performance of downstream applications such as transfection of robust cells such as HEK293, 

restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations.

 

 

PD1411-00          Plasmid Midi Kit I             2 Preps

PD1411-01          Plasmid Midi Kit I            10 Preps

PD1411-02          Plasmid Midi Kit I             25 Preps

 

 

* Buffer A1, B1, C1, KB, Elution Buffer, RNase A, ezBindTM Columns, User Manual