JO-FI™ competent cells

 

High efficiency competent cells 

High efficiency competent cells suitable for any type of cloning method and standard plasmid DNA transformation. Stable replication of high-copy plasmids.

 

1.  Advantages of the product

High transformation efficiency: 5–6 x 10⁹ cfu/µg pUC19 DNA 

2.  Description

3.  Comparison of transformation efficiency

Transformation with a high-copy PUC19 plasmid (1 ng) and 50µl of competent cells.

JO-FI™	TOP 10 f'

3-1.  Ligation results

Ligation mixture contained as follows: The pUC19 vector digested with restrictases at the lacZ gene; The insert fragment with compatible DNA ends digested with the same restrictases as vector molecule. Both DNA frafments were mixed at the vector to insert molar ratio of 1:3 and subsequent ligation / transformation performed accordingly to standard procedure. The blue/white recombinant selection was performed by speading out and cultivation of ligation mixture with competent cells on agar media supplemented with X-Gal and IPTG.

3-2.  Cohesive ends

JO-FI™	TOP 10 f'

 

3-3.  Blunt ends

JO-FI™	TOP 10 f'

•   Tetracycline (15 μg/ml) and Streptomycin (25 μg/ml) resistance. 

 

•   The lacIq repressor enables tight, IPTG -dependent  expression control from the trc, tac and lac promoters. 

 

•   The lacZΔM15 deletion allows the white-blue color selection of recombinant clones (LA + X-Gal). 

 

•   recA1 gene mutation reduces the occurrence of undesired recombination events during cloning. 

 

•   endA1 gene mutation decreases endonuclease I activity. This results in a lower level of DNA degradation,

    providing faster plasmid DNA transfer. 

 

•   mcrA gene mutation allows efficient transformation of methylated genomic DNA. 

 

•   hsdR gene mutation allows efficient transformation of unmethylated DNA after PCR amplification. 

 

1033-20            JO-FI™ competent cells            20 pcs

1033-20            JO-FI™ competent cells            40 pcs

Manual