Liga5™

5-minute Ligation Kit

•  Fast, 5-minute procedure

•  For sticky-end or blunt-end DNA

•  Up to 10 times higher yield compared to traditional ligation methods 

Description

The Liga5™ kit contains a T4 phage DNA Ligase that catalyzes the formation

of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl 

termini in duplex DNA or RNA with blunt or cohesive end termini. 

The enzymerepairs single stranded nicks in duplex DNA, RNA or DNA-RNA

hybrids but has no activity on single stranded nucleic acids. The T4 phage

ligase is purifiedfrom an E. coli bacterial strain carrying a plasmid with 

cloned gene g30 of bacteriophage T4. 

1108-25            Liga5™            25 reactions 

1108-50            Liga5™            50 reactions 

1108-150           Liga5™           150 reactions 

Manual

MSDS