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EcoPlate™
제품명: EcoPlate™
용도: Metabolism of Microbial Communities
메이커: Biolog
카달로그: 다운로드


소개

 

EcoPlate™

 

A Rapid, Community-Level Assessment Tool for Studying Microbial Community Dyanamics 

 


•  Measure the metabolism of 31 carbon sources per assayEcoPlate.PNG

•  Each assay panel tests in triplicate

•  Simple colorimetric readout

•  Readable with any microplate reader 


Microbial communities provide useful information about environmental change. Microorganisms are present in virtually all environments andare typically the first organisms to react to chemical and physical changes in the environment. Changes in microbial communities are often a precursor to changes in the health and viability of the environment as a whole. 


Created in accord with recommendations of microbial ecologists specifically for community analysis and microbial ecological studies.Biolog EcoPlates have been found to provide a sensitive and reliable index of environmental change.


This approach, called community-level physiological profiling, has been demonstrated to be effective at distinguished spatial and temporal changes inmicrobial communities. In applied ecological research, the MicroPlates are used as both an assay of the stability of a normal population and to detectand assess changes following the onset of an environmental variable.

 

 


EcoPlate Applications (Incld. Aerobic Microbiome Analysis)

 


Applications.PNG

 

 


TYPICAL PROCEDURE


STEP 1:  Environmental samples are inoculated directly into Biolog MicroPlates either as 

           aqueous samples or after suspension (soil, sludge, sediment, etc…).Ecoplate.png

STEP 2:  The Biolog MicroPlates are incubated and analyzed at defined time intervals.

STEP 3:  The community-level physiological profile is assessed for key characteristics :

           o Pattern development (similarity)

           o Rate of color change in each well

           o Richness of well response (diversity) 

 

Formation of purple color occurs when the microbes can utilize the carbon source and begin to respire. The respiration of the cells in the community reduces a tetrazolium dye that is included with the carbon source.


The reaction patterns are most effectively analyzed with a microplate reader, using the MicroStation System. However, any good microplate reader can be used to provide optical density (OD590) values.


Statistical analysis of the data is typically performed using standard software packages. 

사양


Layout of EcoPlate.PNG

동영상

주문정보

1506    EcoPlate™ – microplate with 3 sets of the same 31 carbon sources   10 Plates / Box

관련자료

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