CAS9 protein

 

More Efficient Gene Editing with Fewer Off Target Effects 

 

 

 

Cas9 is the nuclease component of the CRISPR/Cas9 system

•   Isolated from S. pyogenes 

•   Supplied at 10 µg/µl in a glycerol stock solution to avoid freeze/thaw

    related degradation 

•   Protein format is more efficient than plasmid or RNA-based systems 

•   2 Nuclear localization signals (NLS) for increased nuclear transport and

    efficiency 

•   Fewer off target effects than plasmid-delivered Cas9

 

 

 

1.  More efficient than plasmid DNA Cas9 

T7E1 assay demonstrating cutting efficiency at target and off target sites. 

 

Adapted from Sojung Kim et al. Genome Res. 2014;24:1012-1019

 

 

 

 

 

 

 

 

2.  Faster editing with Cas9 protein vs. plasmid 

Cas9 protein is fully active within 24 hours of electroporation vs. 

3 days with plasmid DNA. 

 

Adapted from Sojung Kim et al. Genome Res. 2014;24:1012-1019

 

 

 

 

3.  Faster degradation = fewer off target effects  

 

 

Cas9 protein is fully degraded approximately 24 hours following 

electroporation vs. >3 days with plasmid DNA. 

Residual Cas9 expression has been proposed as an 

 

important source of off target effects.

 

Adapted from Sojung Kim et al. Genome Res. 2014;24:1012-1019

 

 

 

 

CAS9 Protein        50µg         Supplied at 10µg/µl

CAS9 Protein        250µg             Supplied at 10µg/µl

        

Manual       Certificate of Analysis         SDS