Product
소개
miRNA CRISPR Knockout Kits
Unique dual-gRNA system allows for the complete excision of miRNAs
• All-in-one dual-gRNA CRISPR-Cas9 plasmids
Single guide RNAs are typically used when generating gene knockouts with the CRISPR-Cas9 system. This system introduces double-strand breaks, which the cell attempts to repair with non homologous end joining (NHEJ). NHEJ can be imperfect, leading to small insertions and deletions, which can cause frameshift mutations and the introduction of early stop codons.
It is the introduction of these early stop codons that leads to gene knockout.Because of the reliance on the generation of early stop codons, single guide RNA systems are not effective when knocking out noncoding RNAs, including miRNA.
To address this challenge, Nawgen has developed a dual-gRNA construct system to target flanking genomic regions of the miRNA gene, leading to complete excision of the miRNA gene.
This approach has many advantages:
1. Complete loss of function of the miRNA gene
2. Provides a larger target area to select potent gRNAs
3. Large deletions can be conveniently detected and confirmed by
genomic PCR instead of the more laborious T7 endonuclease assay
The miRNA CRISPR KO Kits–Powered by Nawgen–include a plasmid containing dual CRISPRs for complete excision of miRNA. The plasmid is all-in-one and includes everything needed to excise your miRNA of interest–dual CRISPRs targeting up- and downstream ends of the miRNA as well as Cas9 nuclease.
주문정보
NAW003 miRNA CRISPR Knockout Kits 1 Kit
Included :
1 µg of all-in-one plasmid containing:
- Dual CRISPRs, targeting both the 3′ and 5′ ends of your miRNA of interest
- Cas9 plasmid
Required Information :
1. Species (Ex: Human, Mouse, Rat, Other)
2. Desired miRNA
관련자료