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miRNA CRISPR Knockout Kits
제품명: miRNA CRISPR Knockout Kits
용도: Gene Editing
메이커: Canopy Biosciences
카달로그: 다운로드


소개

 

miRNA CRISPR Knockout Kits

 

Unique dual-gRNA system allows for the complete excision of miRNAs 


 


사진.PNG

•  All-in-one dual-gRNA CRISPR-Cas9 plasmids


•  Cutting-edge bioinformatics algorithm for gRNA design


•  Genome editing can be conveniently confirmed by genomic PCR

 

 

 


             nawgen-crispr.png

 

Single guide RNAs are typically used when generating gene knockouts with the CRISPR-Cas9 system. This system introduces double-strand breaks, which the cell attempts to repair with non homologous end joining (NHEJ). NHEJ can be imperfect, leading to small insertions and deletions, which can cause frameshift mutations and the introduction of early stop codons. 


It is the introduction of these early stop codons that leads to gene knockout.Because of the reliance on the generation of early stop codons, single guide RNA systems are not effective when knocking out noncoding RNAs, including miRNA. 


To address this challenge, Nawgen has developed a dual-gRNA construct system to target flanking genomic regions of the miRNA gene, leading to complete excision of the miRNA gene. 


 

 

crispr_dual_sgrna_cas9-all-in-one-plasmid-300x198.jpg

This approach has many advantages: 


 1.  Complete loss of function of the miRNA gene

 

 2.  Provides a larger target area to select potent gRNAs

 

 3.  Large deletions can be conveniently detected and confirmed by 

     genomic PCR instead of the more laborious T7 endonuclease assay

 


 

 

The miRNA CRISPR KO Kits–Powered by Nawgen–include a plasmid containing dual CRISPRs for complete excision of miRNA. The plasmid is all-in-one and includes everything needed to excise your miRNA of interest–dual CRISPRs targeting up- and downstream ends of the miRNA as well as Cas9 nuclease.  

주문정보

NAW003          miRNA CRISPR Knockout Kits          1 Kit

 

 

Included :

1 µg of all-in-one plasmid containing:

 -  Dual CRISPRs, targeting both the 3′ and 5′ ends of your miRNA of interest

 -  Cas9 plasmid  


Required Information :

1.  Species (Ex: Human, Mouse, Rat, Other)

2.  Desired miRNA

관련자료