TUNR Flexible Gene Editing for Targeted Transgenes

  

The TUNR Targeted Transgenic kit enables you to insert your gene of interest under TUNR control into the AAVS1 locus. AAVS1 is a “safe harbor”—a safe place to integrate transgenes, allowing predictable expression from a locus known not to alter the expression of other genes or have otherwise deleterious effects on the cell.

 

 

•  Precisely tune gene expression from 100% all the way down to complete

  knockout 

•  Model ranges of gene expression reflecting diversity in patient populations 

•  Control endogenous gene expression–eliminate artifacts from ectopic 

  expression 

•  Knockdown essential genes where knockout would result in lethality

  

 

How the TUNR Flexible Gene Editing System works 

License

Canopy Biosciences holds the exclusive license for TUNR from Washington University and Johns Hopkins University.

 

 

TUNR0005          TUNR Targeted Transgene Kits          1 kit

Controls             Empty Vector Control                     Option   

Controls             Positive Contorl, eGFP                     Option 

 

 

 

The TUNR Targeted Transgenic kit contains everything you need to integrate your gene of interest, 

under TUNR control, into the AAVS1 locus: 

•  crRNA targeting the AAVS1 locus 

•  tracRNA 

•  Cas9 protein 

•  Plasmids, containing one of two TUNR sequences and your target gene, flanked by homology arms to the AAVS1 locus  

   -  TUNR-ML Lower level of repression

   -  TUNR-H Higher level of repression

•  Forward and reverse primers

Required Information 

1.  Species: Human (AAVS1) or Mouse (Rosa26) 

2.  Gene ID 

References

 

Arthur, L. L. et al. Rapid generation of hypomorphic mutations. Nat. Commun. 8, 14112 doi: 10.1038/ncomms14112 (2017). 

Arthur, LL. et al. Translational Control by Lysine-Encoding A-Rich Sequences. Science Advances 1.6 (2015): e1500154 

Koutmou, KS et al. Ribosomes Slide on Lysine-Encoding Homopolymeric A Stretches. Ed. Nahum Sonenberg. eLife 4 (2015): e05534